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首页> 外文期刊>Journal of stroke and cerebrovascular diseases: The official journal of National Stroke Association >Decreased Expression of Cerebral Dopamine Neurotrophic Factor in Platelets of Stroke Patients
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Decreased Expression of Cerebral Dopamine Neurotrophic Factor in Platelets of Stroke Patients

机译：减少脑卒中患者血小板脑多巴胺神经营养因子的表达

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Background: Cerebral dopamine neurotrophic factor plays a critical role in repairing and maintaining healthy neurons in pathological conditions such as stroke. However, the association between cerebral dopamine neurotrophic factor expression and stroke has only recently been investigated in preclinical models and is rarely described in human studies. Objectives: The aims of this were to examine neurological alterations mirrored in human blood platelet cerebral dopamine neurotrophic factor gene expression. Cerebral dopamine neurotrophic factor is expressed in both the central nervous system and peripheral blood. Blood platelets are often used to model neuronal behavior because they exhibit biochemical impairments similar to brain tissues of patients with neurological disorders. Methods: RNA was isolated from platelets and cDNA was synthesized to quantify cerebral dopamine neurotrophic factor gene expression of 36 stroke patients compared to 72 healthy agedmatched controls through real-time PCR. Further grouping analyses of data with regard to age, sex, and medication history were performed. Results: Cerebral dopamine neurotrophic factor gene expression was significantly reduced in stroke patients relative to control subjects (P = .013). Subsequent analysis revealed a significant difference in expression between males and females within the control group (P = .026). Decreased cerebral dopamine neurotrophic factor expression was only observed in male stroke patients compared to their sex-matched controls (P = .008). Grouping stroke patients based on their medication history did not significantly alter cerebral dopamine neurotrophic factor gene expression. Conclusions: Further studies investigating cerebral dopamine neurotrophic factor expression could be directed towards the interplay of the central nervous system, hematopoietic derivatives, and utilizing cerebral dopamine neurotrophic factor as a therapeutic tool.
机译：背景：脑多巴胺神经营养因子在中风等病理状态下修复和维持健康神经元方面起着关键作用。然而，脑多巴胺神经营养因子表达与中风之间的关系直到最近才在临床前模型中进行了研究，很少在人体研究中进行描述。目的：研究人类血小板脑多巴胺神经营养因子基因表达中反映的神经系统改变。大脑多巴胺神经营养因子在中枢神经系统和外周血中均有表达。血小板经常被用来模拟神经元行为，因为它们表现出类似于神经疾病患者脑组织的生化损伤。方法：从血小板中提取RNA，合成cDNA，通过实时PCR定量测定36例脑卒中患者与72例健康对照组的脑多巴胺神经营养因子基因表达。对年龄、性别和用药史方面的数据进行进一步分组分析。结果：与对照组相比，脑卒中患者的大脑多巴胺神经营养因子基因表达显著降低（P=0.013）。随后的分析显示，在对照组中，男性和女性之间的表达存在显著差异（P=0.026）。与性别匹配的对照组相比，仅在男性中风患者中观察到脑多巴胺神经营养因子表达降低（P=0.008）。根据用药史对中风患者进行分组并没有显著改变脑多巴胺神经营养因子基因表达。结论：研究大脑多巴胺神经营养因子表达的进一步研究可针对中枢神经系统、造血衍生物的相互作用，以及利用大脑多巴胺神经营养因子作为治疗工具。

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