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首页> 外文期刊>Journal of the mechanical behavior of biomedical materials >Viscoelastic mapping of mouse brain tissue: Relation to structure and age
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Viscoelastic mapping of mouse brain tissue: Relation to structure and age

机译：小鼠脑组织的粘弹性映射：与结构和年龄的关系

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There is growing evidence that mechanical factors affect brain functioning. However, brain components responsible for regulating the physiological mechanical environment are not completely understood. To determine the relationship between structure and stiffness of brain tissue, we performed high-resolution viscoelastic mapping by dynamic indentation of the hippocampus and the cerebellum of juvenile mice brains, and quantified relative area covered by neurons (NeuN-staining), axons (neurofilament NN18-staining), astrocytes (GFAP-staining), myelin (MBP-staining) and nuclei (Hoechst-staining) of juvenile and adult mouse brain slices. Results show that brain subregions have distinct viscoelastic parameters. In gray matter (GM) regions, the storage modulus correlates negatively with the relative area of nuclei and neurons, and positively with astrocytes. The storage modulus also correlates negatively with the relative area of myelin and axons (high cell density regions are excluded). Furthermore, adult brain regions are similar to 20%-150% stiffer than the comparable juvenile regions which coincide with increase in astrocyte GFAP-staining. Several linear regression models are examined to predict the mechanical properties of the brain tissue based on (immuno)histochemical stainings.
机译：越来越多的证据表明，机械因素会影响大脑功能。然而，负责调节生理机械环境的大脑成分还没有完全被理解。为了确定脑组织结构和硬度之间的关系，我们通过动态压痕幼年小鼠大脑的海马和小脑进行高分辨率粘弹性映射，并量化神经元（NeuN染色）、轴突（神经丝NN18染色）、星形胶质细胞（GFAP染色）覆盖的相对面积，幼年和成年小鼠脑切片的髓鞘（MBP染色）和细胞核（Hoechst染色）。结果表明，脑分区具有明显的粘弹性参数。在灰质（GM）区域，储能模量与细胞核和神经元的相对面积呈负相关，与星形胶质细胞呈正相关。存储模量也与髓鞘和轴突的相对面积呈负相关（高细胞密度区域除外）。此外，成人大脑区域的硬度比青少年区域高20%-150%，这与星形胶质细胞GFAP染色的增加相一致。根据（免疫）组织化学染色，研究了几种线性回归模型来预测脑组织的力学性质。

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