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首页> 外文期刊>Journal of tissue engineering and regenerative medicine >Additive manufacturing of poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate] scaffolds for engineered bone development
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Additive manufacturing of poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate] scaffolds for engineered bone development

机译：聚氯乙烯 - （R）-3-羟基己酸酯的添加剂制造用于工程骨发育的支架

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A wide range of poly(hydroxyalkanoate)s (PHAs), a class of biodegradable polyesters produced by various bacteria grown under unbalanced conditions, have been proposed for the fabrication of tissue-engineering scaffolds. In this study, the manufacture of poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate] (or PHBHHx) scaffolds, by means of an additive manufacturing technique based on a computer-controlled wet-spinning system, was investigated. By optimizing the processing parameters, three-dimensional scaffolds with different internal architectures were fabricated, based on a layer-by-layer approach. The resulting scaffolds were characterized by scanning electron microscopy, which showed good control over the fibre alignment and a fully interconnected porous network, with porosity in the range 79-88%, fibre diameter 47-76 mu m and pore size 123-789 mu m. Moreover, the resulting fibres presented an internal porosity connected to the external fibre surface as a consequence of the phase-inversion process governing the solidification of the polymer solution. Scaffold compressive modulus and yield stress and strain could be varied in a certain range by changing the architectural parameters. Cell-culture experiments employing the MC3T3-E1 murine pre-osteoblast cell line showed good cell proliferation after 21days of culture. The PHBHHx scaffolds demonstrated promising results in terms of cell differentiation towards an osteoblast phenotype. Copyright (c) 2014 John Wiley & Sons, Ltd.
机译：聚羟基烷酸酯（PHA）是一类可生物降解的聚酯，由各种细菌在不平衡条件下生长而成，已被广泛用于组织工程支架的制备。在这项研究中，通过基于计算机控制湿纺系统的添加剂制造技术，研究了聚[（R）-3-羟基丁酸酯-co-（R）-3-羟基己酸]（或PHBHHx）支架的制造。通过优化工艺参数，基于逐层方法制备了具有不同内部结构的三维支架。通过扫描电子显微镜对所得支架进行了表征，扫描电子显微镜显示出对纤维排列的良好控制和完全互连的多孔网络，孔隙率范围为79-88%，纤维直径47-76μm，孔径123-789μm，由于控制聚合物溶液凝固的相变过程，生成的纤维呈现出与外部纤维表面相连的内部孔隙。通过改变结构参数，可以在一定范围内改变支架的压缩模量和屈服应力应变。采用MC3T3-E1小鼠前成骨细胞系的细胞培养实验显示，在培养21天后，细胞增殖良好。PHBHHx支架在向成骨细胞表型的细胞分化方面显示出有希望的结果。版权所有（c）2014约翰威利父子有限公司。

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