Evaluation of a high-throughput nucleic acid extraction method for the detection of Mycobacterium avium subsp. paratuberculosis in bovine fecal samples by PCR

Thirumalapura Nagaraja R.; Feria Willard; Hue Eric; Zellers Corey; Tewari Deepanker

首页> 外文期刊>Journal of Veterinary Diagnostic Investigation >Evaluation of a high-throughput nucleic acid extraction method for the detection of Mycobacterium avium subsp. paratuberculosis in bovine fecal samples by PCR

【24h】

Evaluation of a high-throughput nucleic acid extraction method for the detection of Mycobacterium avium subsp. paratuberculosis in bovine fecal samples by PCR

机译：高通量核酸提取方法检测分枝杆菌亚副亚木分枝杆菌。通过PCR牛粪样品的PALatuburisculosis

获取原文

获取原文并翻译 | 示例

获取外文期刊封面封底 >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Johne's disease (paratuberculosis) is an economically important disease of cattle worldwide. The disease is caused by Mycobacterium avium subsp. paratuberculosis (MAP), a fastidious gram-positive bacterium. PCR is increasingly used in diagnostic laboratories for the detection of MAP in fecal samples given the rapid test turnaround time and sensitivity and specificity comparable to fecal culture. However, efficient extraction of DNA for sensitive detection of MAP by PCR is affected by the complex lipid-rich cell wall of MAP and the presence of PCR inhibitors in feces. We evaluated a high-throughput nucleic acid extraction method (MagMAX core nucleic acid purification kit with mechanical lysis module) in conjunction with an hspX gene PCR for the detection of MAP from bovine fecal samples, which resulted in correct identification of all negative (13 of 13) and positive (35 of 35) proficiency test samples obtained from the National Veterinary Services Laboratories. In addition, all 6 negative and 50 of 51 positive diagnostic specimens tested were categorized correctly.

机译：约翰氏病（副结核）是世界范围内牛的一种重要经济疾病。这种疾病是由鸟分枝杆菌亚种引起的。副结核（MAP），一种挑剔的革兰氏阳性细菌。鉴于快速检测周转时间以及与粪便培养相当的灵敏度和特异性，PCR越来越多地用于诊断实验室检测粪便样本中的MAP。然而，通过PCR敏感检测MAP的有效DNA提取受到MAP复杂的富脂细胞壁和粪便中PCR抑制剂的存在的影响。我们评估了高通量核酸提取方法（带有机械裂解模块的MagMAX核心核酸纯化试剂盒）与hspX基因PCR相结合，用于检测牛粪便样本中的MAP，从而正确识别了从国家兽医服务实验室获得的所有阴性（13个中的13个）和阳性（35个中的35个）能力验证样本。此外，51份阳性诊断标本中的6份阴性和50份被正确分类。

著录项

来源
《Journal of Veterinary Diagnostic Investigation》 |2021年第2期|共4页
作者
Thirumalapura Nagaraja R.; Feria Willard; Hue Eric; Zellers Corey; Tewari Deepanker;
展开▼
作者单位

Penn Dept Agr Penn Vet Lab 2305 North Cameron St Harrisburg PA 17110 USA;

Penn Dept Agr Penn Vet Lab 2305 North Cameron St Harrisburg PA 17110 USA;

Penn Dept Agr Penn Vet Lab 2305 North Cameron St Harrisburg PA 17110 USA;

Penn Dept Agr Penn Vet Lab 2305 North Cameron St Harrisburg PA 17110 USA;

Penn Dept Agr Penn Vet Lab 2305 North Cameron St Harrisburg PA 17110 USA;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类动物医学（兽医学）;
关键词
DNA extraction; feces; Johneamp; 8217; s disease; Mycobacterium avium subsp; paratuberculosis; paratuberculosis; PCR;

机译：DNA提取;粪便;约翰斯;8217;梅毒病;鸟分枝杆菌亚种;副结核;副结核;PCR;

相似文献

外文文献
中文文献
专利

1. Evaluation on the Detection of Mycobacterium Tuberculosis in Clinical Samples by PCR and DNA Probe [J] . 吴雪琼, 庄玉辉, 张晓刚, 中国医学科学杂志：英文版 . 1996,第004期
2. A New High-throughput Real-time PCR Assay for the Screening of Multiple Antimicrobial Resistance Genes in Broiler Fecal Samples from China [J] . CHE Jie, LU Jin Xing, LI Wen Ge, 生物医学与环境科学（英文版） . 2019,第012期
3. Detection of Phthalate Esters in Environmental Water Samples -Comparison of Nylon6 Nanofibers Mat-based Solid Phase Extraction and Other Conventional Extraction Methods [J] . Xu Qian, Yin Xueyan, Shen Yanyan, 中国化学：英文版 . 2011,第003期
4. Detection of Phthalate Esters in Environmental Water Samples-Comparison of Nylon6 Nanofibers Mat-based Solid Phase Extraction and Other Conventional Extraction Methods [J] . Xu Qian, Yin Xueyan, Shen Yanyan, 中国化学（英文版） . 2011,第003期
5. Comparison of Real-Time, Quantitative PCR with Molecular Beacons to Nested PCR and Culture Methods for Detection of Mycobacterium avium subsp. paratuberculosis in Bovine Fecal Samples [J] . Ying Fang, Wai-Hong Wu, Jessica L. Pepper, Journal of Clinical Microbiology . 2002,第1期

机译：实时定量PCR与分子信标，巢式PCR和培养方法检测鸟分枝杆菌亚种的比较。牛粪便样本中的副结核病
6. Improvement of sensitivity for Mycobacterium avium subsp. paratuberculosis (MAP) detection in bovine fecal samples by specific duplex F57/IC real-time and conventional IS900 PCRs after solid culture enrichment [J] . Fawzy Ahmad, Eisenberg Tobias, El-Sayed Amr, Tropical Animal Health and Production . 2015,第4期

机译：提高对鸟分枝杆菌亚种的敏感性。固体培养物富集后，通过特定的双工F57 / IC实时和常规IS900 PCR在牛粪便样品中检测肺结核旁（MAP）
7. Evaluation of PMS-PCR technology for detection of Mycobacterium avium subsp. paratuberculosis directly from bovine fecal specimens. [J] . Salgado M., Steuer P., Troncoso E., Veterinary Microbiology . 2013,第3a4期

机译：评价PMS-PCR技术检测鸟分枝杆菌亚种。副结核病直接来自牛粪标本。
8. Quantitative Real-Time PCR for the detection of Mycobacterium avium subsp. paratuberculosis in bovine fecal samples and liquid culture samples [C] . Sweeney RW, Mangold BL, McAdams S, International Colloquium on Paratuberculosis . 2007

机译：用于检测分枝杆菌亚空间的定量实时PCR。牛粪样品和液体培养样品的副植物
9. Fluorescent-based PCR detection of Mycobacterium avium subsp. paratuberculosis in bovine feces and meat. [D] . Jaravata, Carmela Villanueva. 2006

机译：鸟分枝杆菌亚种的基于荧光的PCR检测。牛粪和肉中的副结核病。
10. Comparison of Real-Time Quantitative PCR with Molecular Beacons to Nested PCR and Culture Methods for Detection of Mycobacterium avium subsp. paratuberculosis in Bovine Fecal Samples [O] . Ying Fang, Wai-Hong Wu, Jessica L. Pepper, 2002

机译：实时定量PCR与分子信标巢式PCR和培养方法检测鸟分枝杆菌亚种的比较。牛粪便样本中的副结核病
11. Comparison of Real-Time, Quantitative PCR with Molecular Beacons to Nested PCR and Culture Methods for Detection of Mycobacterium avium subsp. paratuberculosis in Bovine Fecal Samples [O] . Fang, Ying, Wu, Wai-Hong, Pepper, Jessica L., 2002

机译：实时定量PCR与分子信标，巢式PCR和培养方法检测鸟分枝杆菌亚种的比较。牛粪便样本中的副结核病
12. Final Report Nucleic Acid system PCR, Multiplex Assays and Sample Preparation Project. [R] . Koopman, R. P., Langlois, R. G., Nasaeabadi, S., 2001

机译：最终报告核酸系统pCR，多重分析和样品制备项目。

Evaluation of a high-throughput nucleic acid extraction method for the detection of Mycobacterium avium subsp. paratuberculosis in bovine fecal samples by PCR

摘要

著录项

相似文献

相关主题

期刊订阅