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首页> 外文期刊>Journal of cellular biochemistry. >The power of precise bioinformatics prediction of miRNA:mRNA interactions:miR‐4699 as a potential inducer of Wnt signaling pathway
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The power of precise bioinformatics prediction of miRNA:mRNA interactions:miR‐4699 as a potential inducer of Wnt signaling pathway

机译：miRNA的精确生物信息学预测的功率：mRNA相互作用：miR-4699作为Wnt信号通路的潜在诱导剂

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Abstract microRNAs have attracted interest because of their regulatory effects on gene expression. Experimental detection of potential targets of miRNAs is a laborious task. Considering the expensive techniques of detection, computational approaches for miRNA target prediction can be used as the first step in miRNA research. A large number of tools and algorithms have been developed during the last two decades, led to problems such as confusion in selecting an appropriate tool and false positive or negative results. Therefore, one of the most frequent problems and critical issues of miRNA research is finding a reliable miRNA target prediction tool. In this study, we have proposed a research direction and introduced user‐friendly and current databases and tools with the highest accuracy. To verify whether our proposed research direction is practical, we have provided a case example of predicting a miRNA which can target negative regulators of osteogenesis and experimentally evaluated the accuracy of the prediction results by Real‐Time PCR and Luciferase assay. The results of RT‐qPCR and Luciferase assay indicated a significant decline in expression of Dickkopf‐related protein 1 ( DKK1 ) and tumor necrosis factor ligand superfamily member 11 ( TNFSF11 ) as the key negative regulators of osteogenesis upon overexpression of miR‐4699‐3p. The results emphasize the validity and importance of accurate in silico investigation as the first step in experimental studies. This is the first report detailing the prediction and validation of miR‐4699‐3p target genes. We suggest hsa‐miR‐4699 for further investigation as an osteogenic miRNA for therapeutics purposes.
机译：摘要微RNA因其对基因表达的调节作用而引起人们的兴趣。对miRNA潜在靶点的实验检测是一项艰巨的任务。考虑到昂贵的检测技术，miRNA靶点预测的计算方法可以作为miRNA研究的第一步。在过去的二十年中，已经开发了大量的工具和算法，导致了一些问题，比如在选择合适的工具时出现混乱，以及错误的阳性或阴性结果。因此，寻找可靠的miRNA靶点预测工具是miRNA研究中最常见和关键的问题之一。在这项研究中，我们提出了一个研究方向，并以最高的精确度介绍了用户友好的、最新的数据库和工具。为了验证我们提出的研究方向是否切实可行，我们提供了一个预测可靶向成骨负调节因子的miRNA的案例，并通过实时PCR和荧光素酶分析实验评估了预测结果的准确性。RT-qPCR和荧光素酶分析的结果表明，Dickkopf相关蛋白1（DKK1）和肿瘤坏死因子配体超家族成员11（TNFSF11）的表达显著下降，它们是miR-4699-3p过度表达后成骨的关键负性调节因子。这些结果强调了作为实验研究第一步的准确硅调查的有效性和重要性。这是第一份详细介绍miR-4699-3p靶基因预测和验证的报告。我们建议将hsa-miR-4699作为一种用于治疗目的的成骨miRNA进行进一步研究。

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