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首页> 外文期刊>Brain research >The effect of GABAergic neurotransmission on the seizure-related activity of the laterodorsal thalamic nuclei and the somatosensory cortex in a genetic model of absence epilepsy
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The effect of GABAergic neurotransmission on the seizure-related activity of the laterodorsal thalamic nuclei and the somatosensory cortex in a genetic model of absence epilepsy

机译:Gabaergic神经递质对缺乏癫痫遗传模型中脱底丘脑核和躯体感染皮层的癫痫发作相关活性的影响

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摘要

The present study aimed to investigate the alterations of the GABAergic system in the laterodorsal nucleus (LDN) of the thalamus and the somatosensory cortex (SC) in an experimental model of absence seizure. The effects of pharmacological manipulation of both GABA(A) and GABA(B) receptor subunits in the LDN on the generation of spike-wave discharges (SWD) were evaluated. The experiments were carried out in four groups of both WAG/Rij and Wistar rats with 2 and 6 months of age. The expressions of various GABA receptor subunits were studied in the LDN and SC. Furthermore, recordings of unit activity from the LDN and electrocorticography were simultaneously monitored before, during, and after the application of GABA(A) and GABA(B) antagonists in the LDN. The generation of SWD in the older WAG/Rij rats was associated with significant alterations in the expression of GABA(A)R alpha 1, GABA(A)R beta 3, and GABA(B)R2 subunits in the LDN as well as GABA(A)R alpha 1, GABA(A)RP beta, GABA(A)R gamma 2, and GABA(B)R2 subunits in the SC. Furthermore, the occurrence of SWD was associated with a significant reduction of gene expression of GABA(A)R alpha 1 and increase of GABA(A)R beta 3 in the LDN as well as reduction of GABA(A)R alpha 1, GABA(A)R beta 3, GABA(A)R gamma 3, and GABABR2 in the SC. The microionthophoretic application of the GABA(A) antagonist bicuculline resulted in a significant increase in the population firing rate of LDN neurons as well as the mean number and duration of SWD. The application of the GABA(B) antagonist CGP35348 significantly increased the population firing rate of LDN neurons but decreased the mean number of SWD. Our data indicate the regulatory effect of the GABAergic system of the LDN and SC in absence seizures.
机译:本研究旨在探讨失神发作实验模型中丘脑外侧嗅核(LDN)和体感皮层(SC)GABA能系统的变化。评估了LDN中GABA(A)和GABA(B)受体亚单位的药理学操作对棘波放电(SWD)产生的影响。实验在四组年龄分别为2个月和6个月的WAG/Rij和Wistar大鼠中进行。研究LDN和SC中各种GABA受体亚单位的表达。此外,在LDN中应用GABA(A)和GABA(B)拮抗剂之前、期间和之后,同时监测LDN和皮质电图中的单位活动记录。老年WAG/Rij大鼠SWD的产生与LDN中GABA(A)Rα1、GABA(A)Rβ3和GABA(B)R2亚单位的表达以及SC中GABA(A)Rα1、GABA(A)RPβ、GABA(A)Rγ2和GABA(B)R2亚单位的显著改变有关,SWD的发生与LDN中GABA(a)Rα1基因表达的显著降低和GABA(a)Rβ3的增加,以及GABA(a)Rα1、GABA(a)Rβ3、GABA(a)Rγ3、GABA(a)Rγ3的降低有关,GABA(A)拮抗剂荷包牡丹碱的微离子电泳应用导致LDN神经元的群体放电率以及SWD的平均数量和持续时间显著增加。GABA(B)拮抗剂CGP35348的应用显著增加了LDN神经元的群体放电率,但减少了SWD的平均数量。我们的数据表明,LDN和SC的GABA能系统对失神发作具有调节作用。

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