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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >A new modified nutrient media for cultivation of Actinobacteria
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A new modified nutrient media for cultivation of Actinobacteria

机译：一种新的改性营养培养基，用于肌腱菌培养

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The growth of Actinobacteria was evaluated in a new modified nutrient agar media (MNA) supplemented with 1% sodium chloride (NaCl) and compared with the universally accepted media which are under regular use. Thirteen different Actinobacterial isolates were evaluated for their growth in both solid and liquid media in MNA along with four regularly used media ISP-2, kenknight, starch casein and nutrient media. Results indicated that eight isolates (Streptomyces bulli, Streptomyces griseorubens, Streptomyces viridobrunneus, Streptomyces A3, Streptomyces A7, Streptomyces All, Streptomyces A12 and Streptomyces A13), out of thirteen isolates could grow significantly faster on MNA compared with other solid agar media. Similarly, growth in liquid media recorded by optical density (OD) indicated that three isolates (S. griseorubens, S. viridobrunneus and A7) showed significantly higher OD followed by ISP-2, nutrient, starch casein and kenknight broth after seven days of incubation. All the isolates except S. viridobrunneus and Streptomyces A12 could tolerate NaCl concentration upto 6% in liquid modified nutrient broth. The present study showed that Actinobacteria can be successfully grown faster with full pigmentation at 1% NaCl concentration and they can tolerate up to 5% NaCl concentration, in the new proposed MNA media.
机译：在添加了1%氯化钠（NaCl）的新型改良营养琼脂培养基（MNA）中对放线菌的生长进行了评估，并与常规使用的普遍接受的培养基进行了比较。对13株不同的放线菌菌株在MNA固体和液体培养基以及四种常用培养基ISP-2、kenknight、淀粉酪蛋白和营养培养基中的生长进行了评估。结果表明，与其他固体琼脂培养基相比，13个分离物中的8个（布利链霉菌、灰链霉菌、绿色链霉菌、A3链霉菌、A7链霉菌、All链霉菌、A12链霉菌和A13链霉菌）在MNA上的生长速度显著加快。同样，通过光密度（OD）记录的液体培养基中的生长表明，三个分离物（S.griseorubens、S.viridobrunneus和A7）在培养七天后显示出显著更高的OD，其次是ISP-2、营养素、淀粉酪蛋白和kenknight肉汤。除绿脓杆菌和链霉菌A12外，所有菌株都能耐受液体改性营养肉汤中高达6%的NaCl浓度。目前的研究表明，在新提出的MNA培养基中，放线菌可以在1%NaCl浓度下快速生长，并具有完全色素沉着，它们可以耐受高达5%的NaCl浓度。

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