• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>American Journal of Physiology >Strain-specific differences in lung tissue viscoelasticity of mechanically ventilated infant Sprague-Dawley and Wistar rats
【24h】

Strain-specific differences in lung tissue viscoelasticity of mechanically ventilated infant Sprague-Dawley and Wistar rats

机译：机械通风婴幼婴幼儿和Wistar大鼠肺组织粘弹性的菌株特异性差异

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Rats are often used in ventilator-induced lung injury (VILI) models. However, strain-specific susceptibility for VILI has not been elucidated yet. The aim of this study was to demonstrate strain-specific differences in VILI in infant Sprague-Dawley and Wistar rats. VILI was compared in 2-wk-old pups after 8h of protective or injurious ventilation. Pups were ventilated with tidal volumes (VT) of ~7mL/kg and positive end-expiratory pressures (PEEP) of 6 cmH2O (VT7 PEEP6) or with VT of ~21mL/kg and PEEP 2 cmH2O (VT21 PEEP2). lnterleukin-6, macrophage inflammatory protein-2 (MIP-2), inflammatory cells, and albumin in bronchoalveo-lar lavage fluid (BALF); histology; and low-frequency forced oscillation technique (LFOT) and pressure-volume (PV) maneuvers were assessed. Alveolar macrophages, neutrophils, and MIP-2 derived from BALF revealed more pronounced VILI after VT21 PEEP2 in both strains. LFOT and PV analyses demonstrated rat strain-specific differences both at baseline and particularly in response to VT21 PEEP2 ventilation. Sprague-Dawley rats showed higher airway and tissue resistance and elastance values with no difference in hysteresivity between ventilation strategies. Wister rats challenged by VT21 PEEP2 experienced significantly more energy dissipation when compared with VT7 PEEP6 ventilation. In conclusion, both rat strains are useful for VILI models. The degree of VILI severity depends on ventilation strategy and selected strain. However, fundamental and time-dependent differences in respiratory system mechanics exist and reflect different lung tissue viscoelasticity. Hence, strain-specific characteristics of the respiratory system need to be considered when planning and interpreting VILI studies with infant rats.
机译：大鼠常用于呼吸机诱导的肺损伤（VILI）模型。然而，VILI的菌株特异性易感性尚未阐明。本研究的目的是证明婴儿Sprague Dawley和Wistar大鼠VILI中的菌株特异性差异。在保护性或伤害性通气8小时后，对2周龄幼崽进行VILI比较。幼崽用~7mL/kg的潮气量（VT）和6 cmH2O的呼气末正压（PEEP）（VT7 PEEP6）或~21mL/kg的VT和2 cmH2O的PEEP（VT21 PEEP2）进行通气。支气管肺泡灌洗液（BALF）中白细胞介素-6、巨噬细胞炎性蛋白-2（MIP-2）、炎性细胞和白蛋白；组织学评估了低频强迫振荡技术（LFOT）和压力-容积（PV）机动。在两种菌株中，肺泡巨噬细胞、中性粒细胞和BALF衍生的MIP-2在VT21 PEEP2后显示出更明显的VILI。LFOT和PV分析显示，在基线检查时，尤其是在VT21 PEEP2通气时，大鼠的品系特异性差异。Sprague-Dawley大鼠表现出较高的气道和组织阻力以及弹性值，两种通气策略之间的滞后性没有差异。与VT7 PEEP6通气相比，接受VT21 PEEP2通气的Wister大鼠的能量消耗显著增加。总之，这两种大鼠品系都适用于VILI模型。VILI的严重程度取决于通风策略和选择的菌株。然而，呼吸系统力学存在根本性和时间依赖性差异，反映了不同的肺组织粘弹性。因此，在规划和解释幼鼠VILI研究时，需要考虑呼吸系统的应变特性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号