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Analytical Approaches for Size and Mass Analysis of Large Protein Assemblies

机译:大型蛋白质装配体的尺寸和质量分析的分析方法

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摘要

Analysis of the size and mass of nanoparticles, whether they are natural biomacromolecular or synthetic supramolecular assemblies, is an important step in the characterization of such molecular species. In recent years, electrospray ionization (ESI) has emerged as a technology through which particles with masses up to 100 MDa can be ionized and transferred into the gas phase, preparing them for accurate mass analysis. Here we review currently used methodologies, with a clear focus on native mass spectrometry (MS). Additional complementary methodologies are also covered, including ion-mobility analysis, nanomechanical mass sensors, and charge-detection MS. The literature discussed clearly demonstrates the great potential of ESI-based methodologies for the size and mass analysis of nanoparticles, including very large naturally occurring protein assemblies. The analytical approaches discussed are powerful tools in not only structural biology, but also nanotechnology.
机译:纳米颗粒的大小和质量的分析,无论是天然的生物大分子组装体还是合成的超分子组装体,都是表征此类分子种类的重要步骤。近年来,电喷雾电离(ESI)作为一种技术得以出现,通过该技术可以将质量高达100 MDa的粒子电离并转移到气相中,从而为进行精确的质量分析做好准备。在这里,我们将回顾当前使用的方法,重点明确地放在本地质谱(MS)上。还涵盖了其他补充方法,包括离子迁移率分析,纳米机械质量传感器和电荷检测MS。所讨论的文献清楚地证明了基于ESI的方法学在纳米粒子(包括非常大的天然蛋白质装配体)的尺寸和质量分析方面的巨大潜力。讨论的分析方法不仅是结构生物学的强大工具,而且还是纳米技术的强大工具。

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