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首页> 外文期刊>Annual Review of Analytical Chemistry >Mass Spectrometric Analysis of Histone Proteoforms
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Mass Spectrometric Analysis of Histone Proteoforms

机译：组蛋白形式的质谱分析

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Histones play important roles in chromatin, in the forms of various post-translational modifications (PTMs) and sequence variants, which are called histone proteoforms. Investigating modifications and variants is an ongoing challenge. Previous methods are based on antibodies, and because they usually detect only one modification at a time, they are not suitable for studying the various combinations of modifications on histones. Fortunately, mass spectrometry (MS) has emerged as a high-throughput technology for his-tone analysis and does not require prior knowledge about any modifications. From the data generated by mass spectrometers, both identification and quantification of modifications, as well as variants, can be obtained easily. On the basis of this information, the functions of histones in various cellular contexts can be revealed. Therefore, MS continues to play an important role in the study of histone proteoforms. In this review, we discuss the analysis strategies of MS, their applications on histones, and some key remaining challenges.
机译：组蛋白以各种翻译后修饰（PTM）和序列变体的形式在染色质中发挥重要作用，称为组蛋白蛋白形式。研究修改和变体是一个持续的挑战。先前的方法基于抗体，并且由于它们通常一次只能检测一种修饰，因此它们不适合研究组蛋白修饰的各种组合。幸运的是，质谱（MS）已成为一种用于组蛋白分析的高通量技术，不需要任何修改的先验知识。根据质谱仪生成的数据，可以轻松获得修饰以及变体的鉴定和定量。基于该信息，可以揭示组蛋白在各种细胞环境中的功能。因此，MS在组蛋白的研究中继续发挥重要作用。在本文中，我们讨论了质谱的分析策略，其在组蛋白上的应用以及一些关键的挑战。

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