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Analytical Aspects of Hydrogen Exchange Mass Spectrometry

机译：氢交换质谱的分析方面

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This article reviews the analytical aspects of measuring hydrogen exchange by mass spectrometry (HX MS). We describe the nature of analytical selectivity in hydrogen exchange, then review the analytical tools required to accomplish fragmentation, separation, and the mass spectrometry measurements under restrictive exchange quench conditions. In contrast to analytical quantitation that relies on measurements of peak intensity or area, quantitation in HX MS depends on measuring a mass change with respect to an undeuterated or deuterated control, resulting in a value between zero and the maximum amount of deuterium that can be incorporated. Reliable quantitation is a function of experimental fidelity and to achieve high measurement reproducibility, a large number of experimental variables must be controlled during sample preparation and analysis. The method also reports on important qualitative aspects of the sample, including conformational heterogeneity and population dynamics.
机译：本文回顾了通过质谱法（HX MS）测量氢交换的分析方面。我们描述了氢交换中分析选择性的性质，然后回顾了在限制性交换淬灭条件下完成裂解，分离和质谱测量所需的分析工具。与依赖于峰强度或峰面积测量的分析定量相反，HX MS中的定量取决于测量相对于未氘代或氘代对照的质量变化，从而得出介于零与最大掺入氘之间的值。 。可靠的定量是实验保真度的函数，并且要实现高测量重现性，必须在样品制备和分析过程中控制大量实验变量。该方法还报告了样品的重要定性方面，包括构象异质性和种群动态。

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