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首页> 外文期刊>Annual Review of Analytical Chemistry >Carbon Substrates: A Stable Foundation for Biomolecular Arrays
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Carbon Substrates: A Stable Foundation for Biomolecular Arrays

机译：碳底物：生物分子阵列的稳定基础

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摘要

Since their advent in the early 1990s, microarray technologies have developed into a powerful and ubiquitous platform for biomolecular analysis. Microarrays consist of three major elements: the substrate upon which they are constructed, the chemistry employed to attach biomolecules, and the biomolecules themselves. Although glass substrates and silane-based attachment chemistries are used for the vast majority of current microarray platforms, these materials suffer from severe limitations in stability, due to hydrolysis of both the substrate material itself and of the silyl ether linkages employed for attachment. These limitations in stability compromise assay performance and render impossible many potential microarray applications. We describe here a suite of alternative carbon-based substrates and associated attachment chemistries for microarray fabrication. The substrates themselves, as well as the carbon-carbon bond-based attachment chemistries, offer greatly increased chemical stability, enabling a myriad of novel applications.
机译：自1990年代初问世以来，微阵列技术已发展成为功能强大且无处不在的生物分子分析平台。微阵列由三个主要元素组成：在其上构建微阵列的基质，用于附着生物分子的化学物质以及生物分子本身。尽管玻璃基板和基于硅烷的附着化学方法已用于大多数当前的微阵列平台，但是由于基板材料本身和用于附着的甲硅烷基醚键的水解，这些材料的稳定性受到严重限制。稳定性方面的这些限制损害了测定性能，并使许多潜在的微阵列应用成为不可能。我们在这里描述了一套可供选择的基于碳的基质和用于微阵列制造的相关附着化学方法。基材本身，以及基于碳-碳键的附着化学，都大大提高了化学稳定性，从而实现了许多新颖的应用。

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