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Carvacrol affects breast cancer cells through TRPM7 mediated cell cycle regulation

机译：Carvacrol通过TRPM7介导的细胞周期调节影响乳腺癌细胞

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摘要

As the most prevalent cancer for females, breast cancer is also the second most popular cancer type overall. More efforts are needed to research new drugs and combination therapies for this disease. A naturally derived transient receptor potential melastatin-like 7 channel (TRPM7) inhibitor, carvacrol, was found to have anti-cancer potentials. We hypothesized that carvacrol affects breast cancer cells through TRPM7 mediated cell cycle regulation. Cell viability and apoptosis of breast cancer cell lines BT-483, BT-474, MCF-7, MDA-MB-231, and MDA-MB-453 were determined using the CCK-8 assay and ELISA respectively. TRPM7 in MDA-MB-231, MCF-7 was knocked down. Functional TRPM7 in MDA-MB-231, MCF-7, and HEK293 cells were tested with western blotting, patch-clamp, and fura-2 quench assay. The cell cycle and the regulatory proteins were determined by flow cytometry and western blotting. Results showed that carvacrol inhibited the viability of breast cancer cells with different potency. At 200 mu M, MDA-MB-231 was the most sensitive, and MCF-7 was the least sensitive. At >200 mu M, the apoptosis was dramatically induced. Carvacrol inhibited TRPM7 functions in MDA-MB-231, MCF-7, and HEK293. Carvacrol at 200 mu M increased cells in the G1/G0 phase and decreased cells in the S and G2/M phase by regulating some cyclin proteins in MDA-MB-231. These effects were blocked by the knockdown of TRPM7. This study demonstrated that carvacrol suppresses breast cancer cells by cell cycle regulation and the TRPM7 pathway is one of the pharmacological mechanisms.
机译：乳腺癌是女性最常见的癌症，也是第二大最常见的癌症类型。需要更多的努力来研究治疗这种疾病的新药和联合疗法。一种天然衍生的瞬时受体电位美拉他汀样7通道（TRPM7）抑制剂卡瓦特罗被发现具有抗癌潜力。我们假设香芹醇通过TRPM7介导的细胞周期调节作用于乳腺癌细胞。分别采用CCK-8法和ELISA法检测乳腺癌细胞系BT-483、BT-474、MCF-7、MDA-MB-231和MDA-MB-453的细胞活力和凋亡。MDA-MB-231中的TRPM7，MCF-7被击落。用western印迹、膜片钳和fura-2猝灭试验检测MDA-MB-231、MCF-7和HEK293细胞中功能性TRPM7。流式细胞术和免疫印迹法检测细胞周期和调节蛋白。结果表明，香芹酚以不同的效力抑制乳腺癌细胞的活力。在200μM时，MDA-MB-231最敏感，MCF-7最不敏感。在>200μM时，细胞凋亡被显著诱导。香芹酚抑制MDA-MB-231、MCF-7和HEK293中的TRPM7功能。200μM香芹酚通过调节MDA-MB-231中的一些细胞周期蛋白，使G1/G0期细胞增多，S期和G2/M期细胞减少。这些效应被TRPM7的敲除所阻断。本研究表明，香芹酚通过细胞周期调节抑制乳腺癌细胞，TRPM7通路是其药理机制之一。

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  • 来源
    《Life sciences》 |2021年第1期|共8页
  • 作者

    Li Leilei; He Liang; Wu Yalei; Zhang Yanwu;

  • 作者单位

    Zhengzhou Univ Dept Thyroid &

    Breast Surg Luoyang Cent Hosp Zhengzhou 471009 Peoples R China;

    Zhengzhou Univ Dept Thyroid &

    Breast Surg Luoyang Cent Hosp Zhengzhou 471009 Peoples R China;

    Zhengzhou Univ Dept Thyroid &

    Breast Surg Luoyang Cent Hosp Zhengzhou 471009 Peoples R China;

    Zhengzhou Univ Dept Breast Surg Luoyang Cent Hosp Zhengzhou 471009 Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 医药、卫生;
  • 关键词

    Breast cancer; TRPM7; Cell cycle;

    机译：乳腺癌;TRPM7;细胞周期;

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