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首页> 外文期刊>LWT-Food Science & Technology >Rapid detection for low numbers of Escherichia coli O157:H7 by real-time PCR in cabbage using a combination of filtration, short microbial enrichment, and DNA concentration within 4 h
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Rapid detection for low numbers of Escherichia coli O157:H7 by real-time PCR in cabbage using a combination of filtration, short microbial enrichment, and DNA concentration within 4 h

机译：使用过滤，短的微生物富集和4小时内的DNA浓度的组合，通过白菜实时PCR快速检测较低的大肠杆菌O157：H7

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摘要

Methods: focused on conventional foodborne pathogen detection by selective media are cumbersome and require long time for culture enrichment. Several rapid tests have been developed for the detection of Escherichia coli O157:H7 in the food industry. However, the E. coli O157:H7 method takes very long and requires more than one day to achieve the detection threshold using PCR based enrichment in food. We reported a combination of filtration-based accelerated sample concentration methods to detect <9 CFU/25g of E. coli O157:H7 in cabbage. Filtration, short concentration, as well as DNA concentration contribute to making the detection level the target for real-time PCR analysis. The short enrichment was performed after the filtration, which was shorter than the conventional enrichment method. It was concluded that 0-7 CFU/25 g of E. coli O157:H7 was detected within 4 h after 2 h of incubation following filtration. This combination of filter-based enrichment methods is a faster and more efficient way for reaching the desired bacterial concentration compared to the conventional culture enrichment steps, and can significantly reduce the time required to obtain assay results.
机译：方法：通过选择性培养基进行传统食源性病原体检测比较麻烦，需要很长时间进行培养富集。已经开发了几种快速检测食品工业中大肠杆菌O157:H7的方法。然而，大肠杆菌O157:H7方法需要很长时间，并且需要一天以上的时间才能通过基于PCR的食品浓缩达到检测阈值。我们报道了一种基于过滤的加速样品浓缩方法的组合，用于检测白菜中<9 CFU/25g的大肠杆菌O157:H7。过滤、短浓度以及DNA浓度有助于使检测水平成为实时PCR分析的目标。过滤后进行短时间浓缩，比传统浓缩方法短。结论是，过滤后培养2h后，在4h内检测到0-7cfu/25g大肠杆菌O157:H7。与传统培养富集步骤相比，这种基于过滤器的富集方法的组合是达到所需细菌浓度的更快、更有效的方法，并且可以显著缩短获得分析结果所需的时间。

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