Variable immersion microscopy with a high numerical aperture

Ishida Keita; Naruse Kanta; Mizouchi Yuta; Ogawa Yoshihiro; Matsushita Michio; Shimi Takeshi; Kimura Hiroshi; Fujiyoshi Satoru

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Variable immersion microscopy with a high numerical aperture

机译：具有高数值孔径的可变浸没显微镜

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Three-dimensional (3D) optical microscopy with a high numerical aperture (NA) remains challenging for thick biological specimens owing to aberrations arising from interface refractions. We developed a variable immersion lens (VIL) to passively minimize these aberrations. A VIL is a high-NA concentric meniscus lens and was used in combination with an aberration-corrected high-NA reflecting objective (TORA-FUJI mirror). Wave-optics simulation at a wavelength of 488 nm showed that a VIL microscope enables diffraction-limited 1.2-NA imaging in water (refractive index of 1.34) at a depth of 0.3 mm by minimizing aberrations due to refraction of a sample interface. Another aberration due to the refractive index mismatching between a mounting medium, and an object can also be corrected by the VIL system, because various fluids with different refractive indices can be used as mounting media for the VIL. As a result of correcting the two aberrations at the same time, we experimentally demonstrated that a 6 mu m diameter fluorescent bead can be imaged to the true dimensions in 3D. (C) 2021 Optical Society of America

机译：由于界面折射引起的像差，高数值孔径（NA）的三维光学显微镜对于厚生物样本仍然具有挑战性。我们开发了一种可变浸没透镜（VIL）来被动地最小化这些像差。VIL是一种高NA同心弯月面透镜，与像差校正高NA反射物镜（TORA-FUJI反射镜）结合使用。波长为488 nm的波动光学模拟表明，VIL显微镜通过最小化样品界面折射引起的像差，在0.3 mm深度的水中（折射率为1.34）实现衍射受限的1.2-NA成像。由于安装介质和物体之间的折射率不匹配而产生的另一个像差也可以通过VIL系统进行校正，因为具有不同折射率的各种流体可以用作VIL的安装介质。作为同时校正两个像差的结果，我们实验证明直径为6μm的荧光珠可以在3D中成像到真实尺寸。（2021）美国光学学会

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《Optics Letters》 |2021年第4期|共4页
作者
Ishida Keita; Naruse Kanta; Mizouchi Yuta; Ogawa Yoshihiro; Matsushita Michio; Shimi Takeshi; Kimura Hiroshi; Fujiyoshi Satoru;
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Tokyo Inst Technol Dept Phys Tokyo 1528550 Japan;

Tokyo Inst Technol Dept Phys Tokyo 1528550 Japan;

Tokyo Inst Technol Dept Phys Tokyo 1528550 Japan;

Tokyo Inst Technol Dept Phys Tokyo 1528550 Japan;

Tokyo Inst Technol Dept Phys Tokyo 1528550 Japan;

Tokyo Inst Technol Inst Innovat Res Yokohama Kanagawa 2268503 Japan;

Tokyo Inst Technol Inst Innovat Res Yokohama Kanagawa 2268503 Japan;

Tokyo Inst Technol Dept Phys Tokyo 1528550 Japan;

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正文语种 eng
中图分类计量学;光学;
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Variable immersion microscopy with a high numerical aperture

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