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Investigation of in-vitro antibacterial and seed germination properties of green synthesized pure and nickel doped ZnO nanoparticles

机译:绿色合成纯和镍掺杂ZnO纳米粒子的体外抗菌和种子萌发性能研究

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摘要

A plant mediated, green and cost effective route was utilized for synthesis of pure and Ni-doped Zinc oxide nanoparticles (ZnO-NPs) using Brassica rapa leaf extract. Optical, structural and morphological properties of prepared nanoparticles were affirmed through UV-Vis spectroscopy, X-ray diffraction (XRD) and Scanning electron microscopy (SEM), respectively. The synthesized ZnO-NPs represented hexagonal phase with crystallite size of 27.48 nm and were irregular in shape. The existence of functional groups was explored using Fourier transform infrared spectroscopy. The antibacterial activity of the prepared nanoparticles was verified against a gram positive bacteria Micrococcus luteus and a gram negative bacteria Enterobacter aerogenes which confirmed that ZnO-NPs are efficient antibacterial agents. In addition, the prepared nanoparticles were applied on wheat and pearl millet seeds to investigate their effect on germination. It was observed that there was an appreciable enhancement in seed germination of seeds after treated with the synthesized ZnO-NPs.
机译:采用植物介导、绿色、经济的方法,利用芸苔叶提取物合成了纯的和掺镍的氧化锌纳米颗粒(ZnO NP)。分别通过紫外可见光谱、X射线衍射(XRD)和扫描电子显微镜(SEM)确认了制备的纳米颗粒的光学、结构和形态特性。合成的ZnO纳米颗粒为六方相,晶粒尺寸为27.48nm,形状不规则。利用傅里叶变换红外光谱研究了官能团的存在。制备的纳米颗粒对革兰氏阳性细菌黄体微球菌和革兰氏阴性细菌产气肠杆菌的抗菌活性进行了验证,证实了ZnO纳米粒是有效的抗菌剂。此外,将制备的纳米颗粒应用于小麦和珍珠谷子种子,以研究其对发芽的影响。观察到,经合成的ZnO NP处理后,种子的发芽率显著提高。

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