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Second-Order Nonlinear Optical Imaging of Chiral Crystals

机译:手性晶体的二阶非线性光学成像

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Second-order nonlinear optical imaging of chiral crystals (SONICC) is an emerging technique for crystal imaging and characterization. We provide a brief overview of the origin of second harmonic generation signals in SONICC and discuss recent studies using SONICC for biological applications. Given that they provide near-complete suppression of any background, SONICC images can be used to determine the presence or absence of protein crystals through both manual inspection and automated analysis. Because SONICC creates high-resolution images, nucleation and growth kinetics can also be observed. SONICC can detect metastable, homochiral crystalline forms of amino acids crystallizing from racemic solutions, which confirms Ostwald's rule of stages for crystal growth. SONICC's selectivity, based on order, and sensitivity, based on background suppression, make it a promising technique for numerous fields concerned with chiral crystal formation.
机译:手性晶体的二阶非线性光学成像(SONICC)是一种新兴的晶体成像和表征技术。我们简要概述了SONICC中二次谐波产生信号的来源,并讨论了使用SONICC进行生物学应用的最新研究。鉴于它们可提供几乎所有背景的抑制,因此SONICC图像可用于通过手动检查和自动分析来确定蛋白质晶体的存在与否。由于SONICC可以创建高分辨率图像,因此也可以观察到成核和生长动力学。 SONICC可以检测从外消旋溶液中结晶出来的氨基酸的亚稳态,同手性晶体形式,这证实了奥斯特瓦尔德晶体生长阶段的规律。 SONICC基于顺序的选择性和基于背景抑制的灵敏度,使其成为涉及手性晶体形成的众多领域的有前途的技术。

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