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Vibrational Spectroscopy of Biomembranes

机译:生物膜的振动光谱

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Vibrational spectroscopy, commonly associated with IR absorption and Raman scattering, has provided a powerful approach for investigating interactions between biomolecules that make up cellular membranes. Because the IR and Raman signals arise from the intrinsic properties of these molecules, vibrational spectroscopy probes the delicate interactions that regulate biomembranes with minimal perturbation. Numerous innovative measurements, including nonlinear optical processes and confined bilayer assemblies, have provided new insights into membrane behavior. In this review, we highlight the use of vibrational spectroscopy to study lipid-lipid interactions. We also examine recent work in which vibrational measurements have been used to investigate the incorporation of peptides and proteins into lipid bilayers, and we discuss the interactions of small molecules and drugs with membrane structures. Emerging techniques and measurements on intact cellular membranes provide a prospective on the future of vibrational spectroscopic studies of biomembranes.
机译:通常与红外吸收和拉曼散射有关的振动光谱法为研究组成细胞膜的生物分子之间的相互作用提供了一种有力的方法。因为红外和拉曼信号是由这些分子的固有特性产生的,所以振动光谱法以微扰的方式探测了调节生物膜的微妙相互作用。许多创新的测量,包括非线性光学过程和受限的双层组件,为膜行为提供了新的见解。在这篇综述中,我们重点介绍了使用振动光谱研究脂质-脂质相互作用的方法。我们还检查了最近的工作,其中振动测量已用于研究将肽和蛋白质掺入脂质双层中,并且我们讨论了小分子和药物与膜结构的相互作用。完整细胞膜上的新兴技术和测量为生物膜的振动光谱研究的未来提供了前景。

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