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首页> 外文期刊>Hepatology: Official Journal of the American Association for the Study of Liver Diseases >N-Terminal PreS1 Sequence Regulates Efficient Infection of Cell-Culture-Generated Hepatitis B Virus
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N-Terminal PreS1 Sequence Regulates Efficient Infection of Cell-Culture-Generated Hepatitis B Virus

机译:N末端PRE1序列调节细胞培养生成的乙型肝炎病毒的有效感染

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摘要

Background and AimsAn efficient cell-culture system for hepatitis B virus (HBV) is indispensable for research on viral characteristics and antiviral reagents. Currently, for the HBV infection assay in cell culture, viruses derived from HBV genome-integrated cell lines of HepG2.2.15 or HepAD-38 are commonly used. However, these viruses are not suitable for the evaluation of polymorphism-dependent viral characteristics or resistant mutations against antiviral reagents. HBV obtained by the transient transfection of the ordinary HBV molecular clone has limited infection efficiencies in cell culture.
机译:背景和目的高效的乙型肝炎病毒(HBV)细胞培养系统对于病毒特性和抗病毒试剂的研究是必不可少的。目前,对于细胞培养中的HBV感染检测,来自HBV基因组整合HepG2细胞系的病毒。通常使用2.15或HepAD-38。然而,这些病毒不适合评估依赖多态性的病毒特征或抗病毒试剂的耐药突变。通过瞬时转染普通HBV分子克隆获得的HBV在细胞培养中的感染效率有限。

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