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首页> 外文期刊>American journal of reproductive immunology : >Characterizing the immune cell population in the human fetal membrane
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Characterizing the immune cell population in the human fetal membrane

机译：表征人类胎儿膜中的免疫细胞群体

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Problem: This study localized CD45+ immune cells and compared changes in their numbers between term, not in labor (TNIL) and term, labor (TL) human fetal membranes. Method of study: Fetal membranes (amniochorion) from normal TNIL and TL subjects were analyzed by immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry for evidence of total (CD45+) immune cells as well as innate immune cells (neutrophils, macrophages and NK cells) using specific markers. Fetal origin of immune cells was determined using polymerase chain reaction (PCR) for SRY gene in Y chromosome. Results: CD45+ cells were localized in human fetal membranes for both TNIL and TL. A threefold increase in CD45+ cells was seen in TL fetal membranes of (7.73% ± 2.35) compared to TNIL (2.36% ± 0.78). This increase is primarily contributed by neutrophils. Macrophages and NK cells did not change in the membranes between TNIL and TL. Leukocytes of fetal origin are present in the fetal membranes. Conclusion: The fetal membranes without decidua contain a small proportion of immune cells. Some of these immune cells in the fetal membrane are fetal in origin. There is a moderate increase of immune cells in the fetal membranes at term labor; however, it is unclear whether this is a cause or consequence of labor. Further functional studies are needed to determine their contribution to membrane inflammation associated with parturition.
机译：问题：这项研究局部局部CD45+免疫细胞，并比较其数量的变化，而不是劳动（TNIL）和术语，劳动（TL）人类胎儿膜。研究方法：通过免疫组织化学（IHC），免疫荧光（IF）和流式细胞术分析了来自正常TNIL和TL受试者的胎儿膜（羊膜膜（羊膜），以证明总（CD45+）免疫细胞以及先天性免疫细胞（中性粒细胞，近中性粒细胞）（中性粒细胞）和NK细胞）使用特定标记。使用聚合酶链反应（PCR）在Y染色体中使用聚合酶链反应（PCR）确定免疫细胞的胎儿起源。结果：CD45+细胞位于人类胎儿膜中的TNIL和TL。与TNIL相比，TL胎儿膜（7.73％±2.35）的CD45+细胞增加了三倍（2.36％±0.78）。这种增加主要由中性粒细胞促成。巨噬细胞和NK细胞在TNIL和TL之间没有变化。胎儿起源的白细胞存在于胎儿膜中。结论：没有Decidua的胎儿膜包含一小部分免疫细胞。胎儿膜中的这些免疫细胞中的一些是胎儿的。胎儿分娩时，胎儿膜中免疫细胞的增加;但是，目前尚不清楚这是劳动的原因还是后果。需要进一步的功能研究来确定它们对与分娩相关的膜炎症的贡献。

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