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首页> 外文期刊>Acta Physiologiae Plantarum >Selection and optimization of a high-producing tissue culture of Panax ginseng C. A. Meyer
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Selection and optimization of a high-producing tissue culture of Panax ginseng C. A. Meyer

机译：人参高产组织培养物的选择和优化

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摘要

Biomass growth, ginsenoside and polysaccharide production in different ginseng tissue cultures, including callus culture, adventitious root culture and hairy root culture, were studied, and the active component contents were compared with that of native ginseng roots. The adventitious root culture was confirmed to be a very nice system, which grew fast and contained a rather high content of ginsenosides. Then, the culture conditions of adventitious root culture were optimized. The results showed that salt strength, various sucrose concentrations, ammoniaitrate ratios and phosphate concentrations had significant influences on adventitious roots growth, secondary metabolite and polysaccharide synthesis in ginseng. The best culture conditions for ginsenoside production seemed to be 0.75 salt strength Murashige and Skoog medium, 4% sucrose, 9 mM ammonia to 36 mM nitrate, and 1.25 mM phosphate, while the optimization for polysaccharide accumulation seemed to be 0.75 salt strength, 6% sucrose, 9 mM ammonia to 36 mM nitrate and 3.75 mM phosphate source. Appropriate conditions allowed for a maximum ginsenoside yield of up to 132.90 mg/L and polysaccharide yield of 407.63 mg/L to be obtained after 4 weeks of culture.
机译：研究了不同的人参组织培养物（包括愈伤组织培养，不定根培养和毛状根培养）的生物量生长，人参皂苷和多糖的产生，并与天然人参根进行了比较。不定根培养物被证实是一个非常好的系统，生长迅速并且含有较高的人参皂苷含量。然后，优化了不定根培养的培养条件。结果表明，盐浓度，各种蔗糖浓度，氨/硝酸盐比和磷酸盐浓度对人参不定根生长，次生代谢产物和多糖合成有显着影响。人参皂甙生产的最佳培养条件似乎是0.75盐浓度的Murashige和Skoog培养基，4％蔗糖，9 mM氨水至36 mM硝酸盐和1.25 mM磷酸盐，而多糖积累的最优化似乎是0.75盐强度，6％蔗糖，9 mM氨水至36 mM硝酸盐和3.75 mM磷酸盐源。在适当的条件下，培养4周后，人参皂甙的最大产量可达132.90 mg / L，多糖产量为407.63 mg / L。

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