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首页> 外文期刊>Acta Physiologiae Plantarum >Enhanced salinity tolerance and improved yield properties in Bangladeshi rice Binnatoa through Agrobacterium-mediated transformation of PgNHX1 from Pennisetum glaucum
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Enhanced salinity tolerance and improved yield properties in Bangladeshi rice Binnatoa through Agrobacterium-mediated transformation of PgNHX1 from Pennisetum glaucum

机译：通过农杆菌介导的青草狼尾草PgNHX1转化，提高孟加拉国水稻Binnatoa的耐盐性并提高产量

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摘要

Rice yield is severely affected by high-salt concentration in the vicinity of the plant. In an effort to engineer rice for improved salt tolerance Agrobacterium-mediated transformation of rice cv. Binnatoa was accomplished with the Pennisetum glaucum vacuolar Na+/H+ antiporter gene (PgNHX1) under the constitutive CaMV35S promoter. For the molecular analysis of putative transgenic plants, PCR and RT-PCR were performed. Transgenic rice plants expressing PgNHX1 showed better physiological status and completed their life cycle by setting flowers and seeds in salt stress, while wild-type plants exhibited rapid chlorosis and growth inhibition. Moreover, transgenic rice plants produced higher grain yields than wild-type plants under salt stress. Assessment of the salinity tolerance of the transgenic plants at seedling and reproductive stages demonstrated the potential of PgNHX1 for imparting enhanced salt tolerance capabilities and improved yield.
机译：稻米的产量受植物附近高盐浓度的严重影响。为了工程改造水稻以提高耐盐性，农杆菌介导的水稻简历转化。 Binnatoa是在组成型CaMV35S启动子下用青草狼疮液泡Na + / H +反转运蛋白基因（PgNHX1）完成的。为了进行推定的转基因植物的分子分析，进行了PCR和RT-PCR。表达PgNHX1的转基因水稻植株表现出更好的生理状态，并通过将花和种子置于盐胁迫下来完成其生命周期，而野生型植株则表现出快速的褪绿和生长抑制作用。此外，在盐胁迫下，转基因水稻植物比野生型植物产生更高的谷物产量。对转基因植物在苗期和生殖期的耐盐性的评估表明，PgNHX1具有增强耐盐能力和提高产量的潜力。

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