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首页> 外文期刊>Acta Physiologiae Plantarum >Mannose, but not glucose or sucrose, disturbs actin cytoskeleton in Arabidopsis thaliana leaves
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Mannose, but not glucose or sucrose, disturbs actin cytoskeleton in Arabidopsis thaliana leaves

机译：甘露糖而不是葡萄糖或蔗糖干扰拟南芥叶片中的肌动蛋白细胞骨架

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摘要

A dynamic cytoskeleton able to recognize and respond to both abiotic and biotic stimuli is necessary for the proper functioning of a living cell. The cytoskeleton is involved in cell growth and division, maintenance of cell shape, cytoplasmic streaming, and organelle movements. Numerous studies have focused on the relationships between sugar metabolism, sugar signaling, and the cytoskeleton in yeast and animal cells. Data on such connections in plants are scarce. In the present study we investigated the effects of exogenously delivered sugars on the plant actin cytoskeleton. Detached Arabidopsis thaliana leaves were incubated with sugars for 2 days and the cytoskeleton was visualized using fluorescent-labeled phalloidin. Glucose and sucrose did not influence the pattern of the actin cytoskeleton. In contrast, mannose caused the disappearance of filamentous structures and generated actin foci. The symptoms started to be visible after 24 h of the exposure to mannose. The effect did not occur in Nicotiana tabacum mesophyll cells. This insensitivity was probably due to the presence of phosphomannose isomerase in tobacco cells. Mannose is commonly used as a selection marker for the transformation of plants lacking the enzymes responsible for the metabolism of mannose-6-phosphate. Exposure to this hexose has been linked with DNA fragmentation and a release of cytochrome c from mitochondria. Both responses are treated as features of programmed cell death. However, in our experiments no DNA laddering was observed in mannose-treated Arabidopsis leaves.
机译：能够识别和响应非生物和生物刺激的动态细胞骨架对于活细胞的正常运作是必需的。细胞骨架参与细胞生长和分裂，细胞形状的维持，细胞质流和细胞器运动。许多研究集中在糖代谢，糖信号传导以及酵母和动物细胞中的细胞骨架之间的关系上。关于植物中这种联系的数据很少。在本研究中，我们调查了外源糖对植物肌动蛋白细胞骨架的影响。将分离的拟南芥叶与糖一起温育2天，并使用荧光标记的鬼笔环肽使细胞骨架可视化。葡萄糖和蔗糖不影响肌动蛋白细胞骨架的模式。相反，甘露糖导致丝状结构消失并产生肌动蛋白病灶。暴露于甘露糖24小时后，症状开始可见。在烟草烟草的叶肉细胞中没有发生这种作用。这种不敏感性可能是由于烟草细胞中存在磷酸甘露糖异构酶。甘露糖通常用作转化标记的选择标记，该植物缺少负责6-磷酸甘露糖代谢的酶。己糖的暴露与DNA片段化和线粒体细胞色素c的释放有关。两种反应均被视为程序性细胞死亡的特征。然而，在我们的实验中，在甘露糖处理的拟南芥叶片中未观察到DNA梯状化。

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