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首页> 外文期刊>Acta Physiologiae Plantarum >Calcium-dependent protein kinase gene ZmCPK12 from maize confers tolerance to drought and salt stresses in transgenic plants
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Calcium-dependent protein kinase gene ZmCPK12 from maize confers tolerance to drought and salt stresses in transgenic plants

机译：玉米的钙依赖性蛋白激酶基因ZmCPK12赋予转基因植物耐干旱和盐胁迫的能力

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摘要

Calcium-dependent protein kinases (CDPKs) are important calcium sensors in calcium-mediated signal transductions in plant cells. Increasing evidence shows that CDPKs play vital regulatory roles in abiotic stress responses in plants. We isolated the ZmCPK12 gene encoding a CDPK from maize seedlings. The predicted ZmCPK12 protein contains a typical conserved CDPK structure, a Ser/Thr kinase domain and four EF-hand domains. A ZmCPK12::hGFP fusion protein was localized in the cytoplasm and nucleus when introduced into Arabidopsis mesophyll protoplasts. ZmCPK12 transcription was highly activated by drought and salt in maize seedlings. Isolation of the ZmCPK12 promoter revealed some cis-acting elements that respond to stress. Overexpression of ZmCPK12 improved plant survival rates under drought conditions and enhanced chlorophyll content in transgenic Arabidopsis plants subjected to salt stress. This study revealed that ZmCPK12 is involved in response to environmental stresses, and that ZmCPK12 can be used for genetic improvement in tolerance to environmental stresses of plants.
机译：钙依赖性蛋白激酶（CDPK）是植物细胞中钙介导的信号转导中重要的钙传感器。越来越多的证据表明CDPK在植物的非生物胁迫响应中起着至关重要的调节作用。我们从玉米幼苗中分离了编码CDPK的ZmCPK12基因。预测的ZmCPK12蛋白包含典型的保守CDPK结构，Ser / Thr激酶结构域和四个EF手结构域。当将ZmCPK12 :: hGFP融合蛋白引入拟南芥叶肉原生质体时，它位于细胞质和细胞核中。 ZmCPK12转录被干旱和盐胁迫下的玉米幼苗高度激活。 ZmCPK12启动子的分离揭示了一些对压力有响应的顺式作用元件。 ZmCPK12的过表达提高了干旱条件下植物的存活率，并提高了遭受盐胁迫的转基因拟南芥植物的叶绿素含量。这项研究表明ZmCPK12参与了对环境胁迫的响应，并且ZmCPK12可用于遗传改良植物对环境胁迫的耐受性。

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