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首页> 外文期刊>Acta Physiologiae Plantarum >Expression analysis and functional characterization of a cold-responsive gene COR15A from Arabidopsis thaliana
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Expression analysis and functional characterization of a cold-responsive gene COR15A from Arabidopsis thaliana

机译：拟南芥冷应答基因COR15A的表达分析和功能表征

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The cold-responsive (COR) genes play an important role in cold acclimation of higher plants. Here, a tight correlation between chloroplast functionality and COR15A expression, and the functional characterization of Arabidopsis COR15A involved in salt/osmotic stress, were revealed. COR15A gene is light inducible and expressed in light-grown seedlings. The expression level of COR15A was reduced when chloroplasts were damaged by norflurazon treatment. By using several albino mutants, seca1, secy1, and tic20, all of which exhibited severe defects in both structure and function of chloroplast, it was shown that the accumulation of COR15A mRNA depends on chloroplast functionality. Real-time RT-PCR and GUS-staining assays demonstrated that COR15A was induced by salt/osmotic stress partially via ABA. Overexpression of COR15A in Arabidopsis resulted in the seedlings displaying hypersensitivity to salt/osmotic stress. All these results suggest that plant acquire the ability to fully express COR15A only after the development of functional chloroplasts, COR15A may be involved in response to salt/osmotic stress during early stages of plant development.
机译：冷响应（COR）基因在高等植物的冷驯化中起重要作用。在这里，揭示了叶绿体功能和COR15A表达之间的紧密关系，以及拟南芥COR15A参与盐/渗透胁迫的功能特征。 COR15A基因是光诱导的，并在光生幼苗中表达。当去甲氟氟龙处理破坏叶绿体时，COR15A的表达水平降低。通过使用几个白化突变体seca1，secy1和tic20，它们都在叶绿体的结构和功能上都表现出严重的缺陷，这表明COR15A mRNA的积累取决于叶绿体的功能。实时RT-PCR和GUS染色分析表明，盐/渗透胁迫部分通过ABA诱导COR15A。拟南芥中COR15A的过表达导致幼苗对盐/渗透胁迫表现出超敏性。所有这些结果表明植物仅在功能性叶绿体发育后才具有充分表达COR15A的能力，COR15A可能参与植物发育早期对盐/渗透胁迫的响应。

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