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首页> 外文期刊>Acta Physiologiae Plantarum >Immunogold method evidences that kinesin and myosin bind to and couple microtubules and actin filaments in lipotubuloids of Ornithogalum umbellatum ovary epidermis
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Immunogold method evidences that kinesin and myosin bind to and couple microtubules and actin filaments in lipotubuloids of Ornithogalum umbellatum ovary epidermis

机译：免疫金方法可以证明，驱动蛋白和肌球蛋白结合并结合了虎眼万年青卵巢表皮脂管中的微管和肌动蛋白丝。

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摘要

Lipotubuloids in ovary epidermis of Ornithogalum umbellatum which are a domain of cytoplasm containing a lot of lipid bodies, microtubules and actin filaments, ribosomes, endoplasmic reticulum as well as scarce mitochondria, microbodies, dictyosomes,autolytic vacuoles, exhibit progressive-rotary motion. The immunogold method demonstrated that microtubules and actin filaments of lipotubuloids might be connected with one another by myosin and kinesin. It was supposed that collaboration of motor proteins with actin filaments and microtubules makes autonomic high peripheral speed rotary motion of lipotubuloids in epidermis cells possible. Moreover, myosin was also detected in Golgi bodies in lipotubuloid. In lipotubuloids, the immunogold method demonstrated immunosignals after the use of an antibody to dynein light chains but spectroscopy mass analysis showed that in O. umbellatum epidermis lacked dynein heavy chains.
机译：伞形万年青卵巢表皮中的脂类类化合物是细胞质的一个域，包含大量脂质体，微管和肌动蛋白丝，核糖体，内质网以及稀少的线粒体，微体，单核小体，自溶性液泡，表现出进行性旋转运动。免疫金方法表明，脂质微管的微管和肌动蛋白丝可能通过肌球蛋白和驱动蛋白相互连接。据认为，运动蛋白与肌动蛋白丝和微管的协作使表皮细胞中脂微管的自主性高圆周速度旋转运动成为可能。此外，在脂质微管的高尔基体中也检测到了肌球蛋白。在脂质微管中，免疫金方法在使用针对达因轻链的抗体后证实了免疫信号，但质谱法质谱分析表明在伞形表皮中表皮缺乏达因重链。

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