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首页> 外文期刊>Acta Physiologiae Plantarum >Copy number variations of functional genes influence contents of glycyrrhizic acid in Glycyrrhiza uralensis
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Copy number variations of functional genes influence contents of glycyrrhizic acid in Glycyrrhiza uralensis

机译：功能基因拷贝数变异对甘草甘草酸含量的影响

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摘要

Glycyrrhiza uralensis is a widely used Chinese herb and glycyrrhizic acid is believed to be its marker compound. Three key enzymes, 3-hydroxy-3-methylglutaryl CoA reductase (HMGR), squalene synthase (SQS) and beta-amyrin synthase (beta-AS), are involved in the glycyrrhizic acid biosynthetic pathway. In this paper, the relationship between copy number variations (CNVs) of HMGR, SQS and beta-AS genes and the content levels of glycyrrhizic acid in G. uralensis were investigated. CNVs of the 62 G. uralensis samples from different origins were determined by real-time PCR and their glycyrrhizic acid contents were analyzed by HPLC. The real-time PCR results showed that the copy numbers of HMGR, SQS1 and beta-AS in the 62 G. uralensis samples were either one copy or two copies. According to the copy number patterns of HMGR, SQS1 and beta-AS, the 62 G. uralensis samples can be divided into six groups. Among the six groups, group B with two copies of HMGR, one copy of SQS1 and beta-AS contained relatively higher contents of glycyrrhizic acid. The accumulation of glycyrrhizic acid was lower in the group C with two copies of beta-AS, one copy of SQS1 and HMGR. The results of this work may provide a basis for enhancing the accumulation of glycyrrhizic acid in cultivars of G. uralensis.
机译：甘草是一种广泛使用的中草药，甘草酸被认为是其标志物。甘草酸的生物合成途径涉及到三个关键的酶，3-羟基-3-甲基戊二酰辅酶A还原酶（HMGR），角鲨烯合酶（SQS）和β-淀粉酶合成酶（β-AS）。本文研究了HMGR，SQS和β-AS基因的拷贝数变异（CNV）与甘草中甘草酸含量的关系。通过实时荧光定量PCR测定了62个不同来源的甘草样品的CNV，并通过HPLC分析了其甘草酸的含量。实时荧光定量PCR结果表明，在62个乌拉尔甘草样品中，HMGR，SQS1和β-AS的拷贝数为1个或2个拷贝。根据HMGR，SQS1和beta-AS的拷贝数模式，可将62个G. uralensis样品分为六组。在六组中，具有两个副本的HMGR，一个副本的SQS1和β-AS的B组包含相对较高的甘草酸含量。在具有两个拷贝的β-AS，一个拷贝的SQS1和HMGR的C组中，甘草酸的积累较低。这项工作的结果可为提高甘草酸中甘草酸的积累提供基础。

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