...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta Physiologiae Plantarum >Control of phenylalanine ammonia-lyase gene promoters from pea by UV radiation
【24h】

Control of phenylalanine ammonia-lyase gene promoters from pea by UV radiation

机译：紫外线控制豌豆苯丙氨酸解氨酶基因启动子

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The gene fusion system was used to study UV light-control of PS PAL] and PS PAL2 genes encoding phenylalanine ammonia-lyase of pea. The induction of pea PAL promoters was analysed in transgenic tobacco plants, Binary plasmids (derivatives of pB1101.2 vector) containing 5'regulatory fragments of PS PAL1 and PS PAL2 linked to reporter genes (GUS, LUC) were constructed.The analyses were performed with the use of single constructs (containing one variant of PS PAL promoter and one reporter gene) and dual constructs (containing both PS PAL1 and PS PAL2 promoters connected with different reporter genes). The use of dual constructs enabled the evaluation of both PS PAL promoters activity in the same plant. The analyses of in vitro (grown plants have shown that both PAL promoters are strongly induced in leaves subjected to UV radiation. In some cases, the UV-stimulated expression exceeded the exposed areas. This phenomenon was observed more often in the leaves of plants containing the PS PAL1::GUS than PS PAL2::GUS construct. Removal of boxes 2,4, 5 from PS PAL1 promoter and deletion of its 5'end region (-339 to - 1394) decreases the level of gene expression but does not eliminate its responsiveness to UV.
机译：该基因融合系统用于研究豌豆苯丙氨酸解氨酶的PS PAL1和PS PAL2基因的紫外线控制。在转基因烟草植物中分析了豌豆PAL启动子的诱导，构建了包含与报告基因（GUS，LUC）连接的PS PAL1和PS PAL2 5'调控片段的二元质粒（pB1101.2载体的衍生物）。使用单一构建体（包含PS PAL启动子的一个变体和一个报告基因）和双重构建体（包含与不同报告基因连接的PS PAL1和PS PAL2启动子）。使用双重构建体能够评估同一株植物中两个PS PAL启动子的活性。体外分析（生长中的植物显示，两种PAL启动子均在受到紫外线辐射的叶子中强烈诱导。在某些情况下，受紫外线刺激的表达超过了暴露区域。这种现象在含有以下成分的植物的叶子中更常见） PS PAL1 :: GUS比PS PAL2 :: GUS构建体从PS PAL1启动子上删除框2,4,5并删除其5'末端区域（-339至-1394）会降低基因表达水平，但不会消除其对紫外线的敏感性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号