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首页> 外文期刊>Acta Physiologiae Plantarum >Expression of several genes involved in sucrose/starch metabolism as affected by different strategies to induce phosphate deficiency in Arabidopsis
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Expression of several genes involved in sucrose/starch metabolism as affected by different strategies to induce phosphate deficiency in Arabidopsis

机译：蔗糖/淀粉代谢中涉及的几种基因的表达受拟南芥诱导磷酸盐缺乏的不同策略的影响

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摘要

The effects of inorganic phosphate (Pi) deficiency on expression of genes encoding ADP-glucose pyrophosphorylase small and large subunits (ApS and ApL1, ApL2, ApL3 genes), UDP-glucose pyrophosphorylase (Ugp gene), sucrose synthase (Sus]), Soluble and insoluble acid invertases (Invand Invcw) and hexokinase (Hxk1 gene), all involved in carbohydrate metabolism, were investigated in Arabidopsis thaliana (L.) Heynh. We used soil-grown pho mutants affected in Pi status, as well as wild-type (wt) plants grown under Pi deficiency conditions in liquid medium, and leaves of wt plants fed with D-mannose. Generally, ApS, ApL1, Ugp and Inv genes were upregulated, although to a varied degree, under conditions of Pi-stress. The applied conditions had differential effects on expression of other genes Studied. For instance, Susl was downregulated in phol (Pi-deficient) mutant, but was unaffected in wt plants grown in liquid medium under P-deficiency. Mannose had distinct coil contration-dependent effects on expression of genes under Study, possibly reflecting a dual role of mannose as a sink for Pi and as glucose analog. Feeding- Pi (at up to 200 mM) to the detached leaves of wt plants strongly affected the expression of ApL1, ApL2, Sus1 and Inv genes, possibly due to an osmotic effect exerted by Pi. The data Suggest that ADP-glucose and UDP-glucose pyrophosphorylases (ent, 0 zymes indirectly involved in Pi recycling) as well as invertases (sucrose hydrolysis) are transcriptionally regulated by Pi-deficiency, which may play a role in homeostatic mechanisms that acclimate the plant to the Pi-stress conditions.
机译：无机磷酸盐（Pi）缺乏对编码ADP-葡萄糖焦磷酸化酶大小亚基（ApS和ApL1，ApL2，ApL3基因），UDP-葡萄糖焦磷酸化酶（Ugp基因），蔗糖合酶（Sus）的基因表达的影响在拟南芥（A. Heidh）中研究了与碳水化合物代谢有关的不溶性酸性转化酶（Invand Invcw）和己糖激酶（Hxk1基因）。我们使用了在Pi状况下受影响的土壤生长的pho突变体，以及在液态培养基中在Pi缺乏条件下生长的野生型（wt）植物，以及用D-甘露糖喂养的wt植物的叶子。通常，在Pi胁迫条件下，ApS，ApL1，Ugp和Inv基因上调，尽管程度不同。所施加的条件对所研究的其他基因的表达具有不同的影响。例如，Susl在phol（Pi缺乏）突变体中被下调，但在P缺乏下在液体培养基中生长的wt植物中不受影响。甘露糖对所研究基因的表达具有明显的线圈矛盾依赖性影响，可能反映了甘露糖作为Pi和葡萄糖类似物的沉陷的双重作用。饲喂Pi（至多200 mM）至野生植物的离体叶片，强烈影响ApL1，ApL2，Sus1和Inv基因的表达，这可能是由于Pi发挥了渗透作用。数据表明，ADP葡萄糖和UDP葡萄糖的焦磷酸酶（ent，Pi循环间接参与的0个酶）以及转化酶（蔗糖水解）受到Pi缺乏的转录调控，这可能在适应动物体内的稳态机制中起作用。将植物置于Pi应力条件下。

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