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首页> 外文期刊>APMIS: Acta Pathologica, Microbiologica et Immunologica Scandinavica >The YvqE two-component system controls biofilm formation and acid production in Streptococcus pyogenes
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The YvqE two-component system controls biofilm formation and acid production in Streptococcus pyogenes

机译：YvqE两组分系统控制化脓链球菌的生物膜形成和产酸

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摘要

In Streptococcus pyogenes, proteins involved in determining virulence are controlled by stand-alone response regulators and by two-component regulatory systems. Previous studies reported that, compared to the parental strain, the yvqE sensor knockout strain showed significantly reduced growth and lower virulence. To determine the function of YvqE, we performed biofilm analysis and pH assays on yvqE mutants, and site-directed mutagenesis of YvqE. The yvqE deletion mutant showed a slower acid production rate, indicating that YvqE regulates acid production from sugar fermentation. The mutant strain, in which the Asp(26) residue in YvqE was replaced with Asn, affected biofilm formation, suggesting that this amino acid senses hydrogen ions produced by fermentative sugar metabolism. Signals received by YvqE were directly or indirectly responsible for inducing pilus expression. This study shows that at low environmental pH, biofilm formation in S. pyogenes is mediated by YvqE and suggests that regulation of pilus expression by environmental acidification could be directly under the control of YvqE.
机译：化脓性链球菌中，确定毒力的蛋白质由独立的应答调节剂和两组分调节系统控制。先前的研究报道，与亲本菌株相比，yvqE传感器基因敲除菌株显示出明显降低的生长和较低的毒力。为了确定YvqE的功能，我们对yvqE突变体进行了生物膜分析和pH测定，并对YvqE进行了定点诱变。 yvqE缺失突变体显示出较慢的产酸速度，表明YvqE调节糖发酵产生的酸。突变菌株，其中YvqE中的Asp（26）残基被Asn取代，影响了生物膜的形成，表明该氨基酸可感测发酵糖代谢产生的氢离子。 YvqE收到的信号直接或间接负责诱导菌毛表达。这项研究表明，在低环境pH下，化脓性链球菌的生物膜形成是由YvqE介导的，这表明通过环境酸化对菌毛表达的调节可能直接受YvqE的控制。

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