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首页> 外文期刊>American Journal of Sports Medicine >A new source of mesenchymal stem cells for articular cartilage repair: MSCs derived from mobilized peripheral blood share similar biological characteristics in vitro and chondrogenesis in vivo as MSCs from bone marrow in a rabbit model
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A new source of mesenchymal stem cells for articular cartilage repair: MSCs derived from mobilized peripheral blood share similar biological characteristics in vitro and chondrogenesis in vivo as MSCs from bone marrow in a rabbit model

机译：用于关节软骨修复的间充质干细胞的新来源：动员外周血来源的MSC在体外和体内软骨形成方面与兔模型中的MSC具有相似的生物学特性

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Background: Bone marrow (BM) has been considered as a major source of mesenchymal stem cells (MSCs), but it has many disadvantages in clinical application. However, MSCs from peripheral blood (PB) could be obtained by a less invasive method and be more beneficial for autologous transplantation than BM MSCs, which makes PB a promising source for articular cartilage repair in clinical use. Purpose: To assess whether MSCs from mobilized PB of New Zealand White rabbits have similar biological characteristics in vitro and chondrogenesis in vivo as BM MSCs. Study Design: Controlled laboratory study. Methods: A combined method of drug administration containing granulocyte colony stimulating factor (G-CSF) plus CXCR4 antagonist AMD3100 was adopted to mobilize the PB stem cells of adult New Zealand White rabbits in vitro. The isolated cells were identified as MSCs by morphological characteristics, surface markers, and differentiation potentials. A comparison between PB MSCs and BM MSCs was made in terms of biological characteristics in vitro and chondrogenesis in vivo. This issue was investigated from the aspects of morphology, immune phenotype, multiple differentiation capacity, expansion potential, antiapoptotic capacity, and ability to repair cartilage defects in vivo of PB MSCs compared with BM MSCs. Results: Peripheral blood MSCs were successfully mobilized by the method of combined drug administration, then isolated, expanded, and identified in vitro. No significant difference was found concerning the morphology, immune phenotype, and antiapoptotic capacity between PB MSCs and BM MSCs. Significantly, MSCs from both sources compounded with decalcified bone matrix showed the same ability to repair cartilage defects in vivo. For multipluripotency, BM MSCs exhibited a more osteogenic potential and higher proliferation capacity than PB MSCs, whereas PB MSCs possessed a stronger adipogenic and chondrogenic differentiation potential than BM MSCs in vitro. Conclusion: Although there are some differences in the proliferation and differentiation aspects between the 2 sources, PB MSCs share certain similar biological characteristics in vitro and chondrogenesis in vivo as BM MSCs. Clinical Relevance: These results suggest that PB MSCs are a new source of seed cells used in articular cartilage repair.
机译：背景：骨髓（BM）被认为是间充质干细胞（MSCs）的主要来源，但在临床应用中有许多缺点。但是，外周血（PB）的MSC可以通过侵入性较小的方法获得，并且比BM MSC更有利于自体移植，这使得PB成为临床上有望进行关节软骨修复的来源。目的：评估来自新西兰白兔动员PB的MSCs在体外和体内软骨形成方面是否与BM MSCs相似。研究设计：受控实验室研究。方法：采用含粒细胞集落刺激因子（G-CSF）加CXCR4拮抗剂AMD3100的联合给药方法，体外动员成年新西兰白兔的PB干细胞。通过形态特征，表面标记和分化潜能将分离的细胞鉴定为MSC。在体外生物学特性和体内软骨形成方面，对PB MSC和BM MSC进行了比较。从形态学，免疫表型，多重分化能力，扩增潜能，抗凋亡能力以及修复PB MSCs与BM MSCs体内软骨缺损的能力等方面研究了该问题。结果：通过联合给药方法成功地动员了外周血MSC，然后进行了分离，扩增和体外鉴定。在PB MSC和BM MSC之间在形态，免疫表型和抗凋亡能力方面没有发现显着差异。重要的是，两种来源的MSC与脱钙骨基质复合后，在体内修复软骨缺损的能力均相同。就多能性而言，BM MSCs比PB MSCs具有更多的成骨潜能和更高的增殖能力，而PB MSCs在体外具有比BM MSCs更强的成脂和成软骨分化潜能。结论：尽管这两种来源在增殖和分化方面存在一定差异，但PB MSCs在体外和体内软骨形成方面与BM MSCs具有某些相似的生物学特性。临床意义：这些结果表明，PB MSC是关节软骨修复中使用的种子细胞的新来源。

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