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首页> 外文期刊>Advances in Horticultural Science >Cryopreservation of plant germplasm in Argentina
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Cryopreservation of plant germplasm in Argentina

机译:阿根廷植物种质的超低温保存

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摘要

This review describes the current status of development of methods for cryopreservation (at -196 degrees C) of plants germplasm in Argentina. Arachis pintoi, a forage legume, has been maintained as seeds using vitrification method. Additionally, apical meristems, shoot tips, and somatic embryos have been cryopreserved using encapsulation-dehydration. Zygotic embryos, encapsulated and dehydrated, have permitted the cryopreservation of seven species of the genus Ilex. Various explants (apical meristems, uninodal segments, buds and somatic embryos) of Melia azedarach have been cryopreserved using the encapsulation-dehydration method. Protocols based in encapsulation-dehydration have also been developed for shoot tips of Citrus sinensis, seeds and protocorms of Oncidum bifolium and anthers of Oryza sativa. Vitrification protocols have been developed for cryopreservation of shoot tips of Solanum tuberosum and seeds of Toona ciliata.
机译:这篇综述描述了阿根廷植物种质的低温保存(-196摄氏度)方法的发展现状。饲用豆科植物花生种子已经使用玻璃化方法作为种子保存。另外,已经使用包囊-脱水冷冻保存了顶端分生组织,茎尖和体细胞胚。合子胚经封装和脱水后,可以冷冻保存Ilex属的7种。使用封装-脱水方法冷冻保存了Mel子花的各种外植体(顶端分生组织,单节节,芽和体细胞胚)。还开发了基于包囊-脱水的方案,用于柑橘的梢尖,大头菜Oncidum bifolium的种子和原球茎以及水稻的花药。已经开发了玻璃化方案用于冷冻保存马铃薯的茎尖和纤毛香椿的种子。

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