Genetic Manipulation of Vibrio fischeri

David G. Christensen; Jovanka Tepavcevic; Karen L. Visick

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Genetic Manipulation of Vibrio fischeri

机译：费氏弧菌的遗传操作

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摘要

Vibrio fischeri is a nonpathogenic organism related to pathogenic Vibrio species. The bacterium has been used as a model organism to study symbiosis in the context of its association with its host, the Hawaiian bobtail squid Euprymna scolopes. The genetic tractability of this bacterium has facilitated the mapping of pathways that mediate interactions between these organisms. The protocols included here describe methods for genetic manipulation of V. fischeri. Following these protocols, the researcherwill be able to introduce linear DNA via transformation to make chromosomal mutations, to introduce plasmid DNA via conjugation and subsequently eliminate unstable plasmids, to eliminate antibiotic resistance cassettes from the chromosome, and to randomly or specifically mutagenize V. fischeri with transposons.

机译：费氏弧菌是不致病的微生物与致病性弧菌的物种。细菌已被用来作为生物模型研究共生的上下文关联主机,夏威夷短尾猫鱿鱼Euprymna scolopes。这种细菌的映射途径调解之间的相互作用生物。诉fischeri的基因操纵的方法。这些协议后,researcherwill能介绍线性DNA通过变换使染色体突变,介绍通过结合随后质粒DNA消除质粒不稳定,消除抗生素耐药性的磁带染色体,随机或特别诱变处理诉fischeri转座子。

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来源
《Current Protocols in Microbiology》 |2020年第1期|Genetic Manipulation/1-Genetic Manipulation/27|共27页
作者
David G. Christensen; Jovanka Tepavcevic; Karen L. Visick;
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作者单位

Department of Biology, Wheaton College, Wheaton, Illinois;

Department of Microbiology and Immunology, Loyola University Chicago, Maywood, Illinois;

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原文格式 PDF
正文语种英语
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关键词
Chromosomes; Transposons; SymbiosisconjugationEliminateAliivibrio fischeriVibrio spbacteriaGenetic Engineering;

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3. Sulfur availability for Vibrio fischeri Vibrio fischeri growth during symbiosis establishment depends on biogeography within the squid light organ [J] . Wasilko Nathan P., Larios‐Valencia Jessie, Steingard Caroline H., Molecular Microbiology . 2019,第3期

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机译：细菌仲裁传感调节在<命名含量含量型=“属型”> euprymna scolopes - <命名内容内容=“属的属中，诱导关键宿主组织的形态变化 “> vibrio fischeri 共生
6. Synergistic and Hermetic Effects of Volatile Organic Compounds on Marine Luminescent Bacterium Vibrio fischeri [C] . Shiro Ikeda, Hideaki Sekine, Yoshika Sekine Annual conference of the International Society of Exposure Science . 2014

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8. Quorum Sensing and Cyclic di-GMP Exert Control Over Motility of Vibrio fischeri KB2B1 [O] . Courtney N. Dial, Steven J. Eichinger, Randi Foxall, 2021

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10. Role of Light Organ Symbiosis in the Distribution and Diversity of the MarineLuminous Bacterium Vibrio fischeri [R] . Ruby, E. G. 1993

机译：光器官共生在海洋光合细菌Vibrio fischeri的分布和多样性中的作用

Genetic Manipulation of Vibrio fischeri

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