Sporulation and Germination of UNIT 9E.2Paenibacillus larvae Cells

Osama S. Mahdi; Nathan A. Fisher

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Sporulation and Germination of UNIT 9E.2Paenibacillus larvae Cells

机译：孢子形成和萌发的单位9 e。

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Endospores are metabolically dormant cells formed by a variety of Grampositivebacteria within the phylum Firmicutes in response to nutrient limitingor otherwise unfavorable growth conditions. American foulbrood disease(AFB) is a serious disease of honeybees that is caused by the introduction ofPaenibacillus larvae endospores into a honeybee colony. Progression to fulminantdisease and eventual collapse of the colony requires multiple rounds ofendospore germination, vegetative replication, endospore formation, and subsequentspread within the colony. This unit includes protocols for the in vitrosporulation and germination of P. larvae to assist investigators in the study ofthese processes.

机译：内孢子是新陈代谢休眠细胞形成由多种Grampositivebacteria内门壁厚菌门,以应对营养limitingor否则不利的增长条件。蜜蜂的严重疾病所致引入ofPaenibacillus幼虫内孢子成蜜蜂殖民地。fulminantdisease和最终的崩溃殖民地需要多轮ofendospore发芽、营养复制内生孢子形成,subsequentspread内殖民地。vitrosporulation和萌发的幼虫协助调查研究这些流程。

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来源
《Current Protocols in Microbiology》 |2018年第1期|共1页
作者
Osama S. Mahdi; Nathan A. Fisher;
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作者单位

Department of Microbiological Sciences, North Dakota State University, Fargo, NorthDakota;

Southern Research Institute, Frederick, Maryland;

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原文格式 PDF
正文语种英语
中图分类
关键词
Germination; Firmicutes; Bacterial SporesPaenibacillus larvaeSporulationLarva;

机译：发芽,厚壁菌门;细菌;

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Sporulation and Germination of UNIT 9E.2Paenibacillus larvae Cells

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