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首页> 外文期刊>Antonie van Leeuwenhoek: Journal of Microbiology and serology >Micromonospora jinlongensis sp nov, isolated from muddy soil in China and emended description of the genus Micromonospora
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Micromonospora jinlongensis sp nov, isolated from muddy soil in China and emended description of the genus Micromonospora

机译：从中国泥泞土壤中分离得到的金龙小单孢菌，并对小单孢菌属的描述进行了改进

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摘要

Abstract: A novel actinomycete, designated strain NEAU-GRX11T, was isolated from muddy soil collected from a stream of Jinlong Mountain in Harbin, north China. The organism was found to have morphological and chemotaxonomic characteristics typical of the genus Micromonospora. The 16S rRNA gene sequence of strain NEAU-GRX11T showed highest similarity to Micromonospora zamorensis CR38T (99.2 %), Micromonospora saelicesensis Lupac 09T (99.0 %), Micromonospora chokoriensis 2-19/6T (98.7 %), Micromonospora coxensis 2-30-b/28T (98.5 %), Micromonospora aurantiaca ATCC 27029T (98.4 %) and Micromonospora lupini lupac 14NT (98.3 %). Phylogenetic analysis based on the 16S rRNA gene and gyrB gene demonstrated that strain NEAU-GRX11T was a member of the genus Micromonospora and supported the closest phylogenetic relationship to M. zamorensis CR38T, M. saelicesensis Lupac 09T, M. chokoriensis 2-19/6T and M. lupini lupac 14NT. A combination of DNA-DNA hybridization and some phenotypic characteristics indicated that the novel strain could be readily distinguished from these closest phylogenetic relatives. Therefore, it is proposed that NEAU-GRX11T represents a novel species of the genus Micromonospora, for which the name Micromonospora jinlongensis sp nov is proposed. The type strain is NEAU-GRX11T (=CGMCC 4.7103 T=DSM 45876T).
机译：摘要：从华北哈尔滨市金龙山溪流中收集的浑浊土壤中分离出一种新的放线菌，命名为NEAU-GRX11T。发现该生物具有微单孢菌属典型的形态和化学分类学特征。 NEAU-GRX11T菌株的16S rRNA基因序列与zamorensis小单孢菌CR38T（99.2％），saelicesensis Lupac 09T小孢子（99.0％），chokoriensis 2-19 / 6T小孢子（98.7％），coxensis 2-30-b的相似性最高。 / 28T（98.5％），微单孢菌aurantiaca ATCC 27029T（98.4％）和微单孢菌lupini lupac 14NT（98.3％）。基于16S rRNA基因和gyrB基因的系统发育分析表明，菌株NEAU-GRX11T是Micromonospora属的一员，并支持与zamorensis CR38T，saelicesensis Lupac 09T，chokoriensis 2-19 / 6T的亲缘关系最近的亲缘关系。和M. lupini lupac 14NT。 DNA-DNA杂交和一些表型特征的结合表明，该新菌株很容易与这些最亲近的系统发育亲戚区分开。因此，提出NEAU-GRX11T代表微单孢菌属的新物种，为此提出了名称金龙微单孢菌。类型菌株为NEAU-GRX11T（= CGMCC 4.7103 T = DSM 45876T）。

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