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首页> 外文期刊>Antonie van Leeuwenhoek: Journal of Microbiology and serology >Streptosporangium lutulentum sp nov., Streptosporangium fenghuangense sp nov and Streptosporangium corydalis sp nov., three novel actinobacterial species isolated from National Forest Park of Fenghuang Mountain
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Streptosporangium lutulentum sp nov., Streptosporangium fenghuangense sp nov and Streptosporangium corydalis sp nov., three novel actinobacterial species isolated from National Forest Park of Fenghuang Mountain

机译:从凤凰山国家森林公园中分离出的三种新型放线菌物种:光链链霉菌,凤凰黄链霉菌和延生链霉菌。

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摘要

Three novel actinobacteria, designated strains NEAU-FSHN1(T), NEAU-hd-3(T) and NEAU-Y6(T), were isolated from a stream base, soil adjacent to the stream and a root of Corydalis yanhusuo L, respectively, collected from Wuchang, Heilongjiang Province, China. A polyphasic study was carried out to establish the taxonomic positions of these strains. The three strains were observed to form scant aerial hyphae that differentiated into spherical spore vesicles. The phylogenetic analysis based on the 16S rRNA gene sequences of strains NEAU-FHSN1(T), NEAU-hd-3(T) and NEAU-Y6(T) showed that the three novel isolates exhibit 99.2 % (NEAU-FHSN1(T)/NEAU-hd-3(T)), 99.2 % (NEAU-FHSN1(T)/NEAU-Y6(T)) and 99.7 % (NEAU-hd-3(T)/NEAU-Y6(T)) 16S rRNA gene sequence similarities with each other and that they are closely related to strains Streptosporangium shengliense NEAU-GH7(T) (sequence similarities 98.72, 98.85, 98.99 %), Streptosporangium roseum DSM 43021(T) (98.65, 98.51, 98.58 %) and Streptosporangium album DSM 43023(T) (98.41, 98.96, 98.89 %). However, the DNA-DNA hybridization values between strains NEAU-FSHN1(T), NEAU-hd-3(T) and NEAU-Y6(T) were 61.2 % (NEAU-FSHN1(T)/NEAU-hd-3(T)), 63.5 % (NEAU-FHSN1(T)/NEAU-Y6(T)) and 65.8 % (NEAU-hd-3(T)/NEAU-Y6(T)), and the values between the three strains and their close phylogenetic relatives were also below 70 %. With reference to phenotypic characteristics, phylogenetic data and DNA-DNA hybridization results, the three strains can be distinguished from each other and their close phylogenetic relatives. Thus, strains NEAU-FHSN1(T), NEAU-hd-3(T) and NEAU-Y6(T) are concluded to represent three novel species of the genus Streptosporangium, for which the names Streptosporangium lutulentum sp. nov., Streptosporangium fenghuangense sp. nov. and Streptosporangium corydalis sp. nov. are proposed. The type strains are NEAU-FHSN1(T) (=CGMCC 4.7141(T) = DSM 46740(T)), NEAU-Y6(T) (=CGMCC 4.7150(T) = DSM 46722(T)) and NEAU-hd3(T) (CGMCC 4.7212(T) = JCM 30058(T)), respectively.
机译:从河床底部,靠近河床的土壤和延胡索的根中分离出三种新的放线菌,分别命名为NEAU-FSHN1(T),NEAU-hd-3(T)和NEAU-Y6(T)。 ,收集自中国黑龙江省武昌市。进行了多相研究以建立这些菌株的分类位置。观察到这三个菌株形成很少的气生菌丝,其分化成球形孢子囊泡。基于菌株NEAU-FHSN1(T),NEAU-hd-3(T)和NEAU-Y6(T)的16S rRNA基因序列进行的系统发育分析表明,这三种新分离株均显示99.2%(NEAU-FHSN1(T)) /NEAU-hd-3(T))、99.2%(NEAU-FHSN1(T)/ NEAU-Y6(T))和99.7%(NEAU-hd-3(T)/ NEAU-Y6(T))16S rRNA基因序列相似性以及它们与菌株Streptosporangium shengliense NEAU-GH7(T)密切相关(序列相似性98.72、98.85、98.99%),玫瑰链霉菌DSM 43021(T)(98.65、98.51、98.58%)和Streptosporangium专辑DSM 43023(T)(98.41,98.96,98.89%)。然而,菌株NEAU-FSHN1(T),NEAU-hd-3(T)和NEAU-Y6(T)之间的DNA-DNA杂交值为61.2%(NEAU-FSHN1(T)/ NEAU-hd-3(T )),63.5%(NEAU-FHSN1(T)/ NEAU-Y6(T))和65.8%(NEAU-hd-3(T)/ NEAU-Y6(T))以及这三株菌株之间的值近亲系统发育亲属也低于70%。参考表型特征,系统发育数据和DNA-DNA杂交结果,可以区分这三个菌株及其亲缘近亲。因此,得出结论,菌株NEAU-FHSN1(T),NEAU-hd-3(T)和NEAU-Y6(T)代表了链霉菌属的三个新种,其名称为链霉菌属lutulentum sp。十一月,链霉菌fenghuangense sp。十一月和链霉菌变种。十一月被提议。类型菌株为NEAU-FHSN1(T)(= CGMCC 4.7141(T)= DSM 46740(T)),NEAU-Y6(T)(= CGMCC 4.7150(T)= DSM 46722(T))和NEAU-hd3( T)(CGMCC 4.7212(T)= JCM 30058(T))。

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