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首页> 外文期刊>Antonie van Leeuwenhoek: Journal of Microbiology and serology >Vulcaniibacterium tengchongense gen. nov., sp. nov. isolated from a geothermally heated soil sample, and reclassification of Lysobacter thermophilus Wei et al. 2012 as Vulcaniibacterium thermophilum comb. nov.
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Vulcaniibacterium tengchongense gen. nov., sp. nov. isolated from a geothermally heated soil sample, and reclassification of Lysobacter thermophilus Wei et al. 2012 as Vulcaniibacterium thermophilum comb. nov.

机译：腾冲旺氏杆菌十一月，sp。十一月从地热加热的土壤样品中分离出来，并对嗜热溶菌进行重新分类Wei等。 2012年以嗜热Vulcaniibacterium thermophilum comb的形式出现。十一月

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摘要

A thermotolerant Gram-staining negative and aerobic bacterium, designated strain YIM 77520T, was isolated from a geothermally heated soil sample collected at Rehai National Park, Tengchong, Yunnan Province, South-West China. Cells of the strain were found to be rod-shaped and colonies were light beige and circular. The strain was found to grow in the presence of 0-2 % (w/v) total salts (optimum, 0 %), at pH 6.0-8.0 (optimum, pH 7.0) and 25-55 C (optimum, 45 C). The only quinone detected was Q-8 and the genomic DNA G+C content was determined to be 66.9 mol%. The major fatty acids (10 %) were identified as iso-C16:0 and iso-C15:0. The phospholipids were found to consist of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, five unknown phospholipids and three aminophospholipids. Based on the 16S rRNA gene sequence analysis, strain YIM 77520T was found to form a cluster with Lysobacter thermophilus YIM 77875T and showed the highest 16S rRNA gene sequence similarity to L. thermophilus YIM 77875T (96.0 %). These two strains formed a distinct lineage of the family 'Xanthomonadaceae'. On the basis of the morphological and chemotaxonomic characteristics, as well as genotypic data, a new genus, Vulcaniibacterium gen. nov. is proposed with Vulcaniibacterium tengchongense sp. nov. as the type species. The type strain of V. tengchongense sp. nov. is strain YIM 77520 T (=DSM 25623T = CCTCC AB 2011152T). Furthermore we propose that L. thermophilus Wei et al. 2012 is reclassified in the new genus as Vulcaniibacterium thermophilum comb. nov. (type strain YIM 77875T = CCTCC AB 2012064T = KCTC 32020T) based on polyphasic data.
机译：从中国云南省腾冲市瑞海国家公园收集的地热土壤样品中分离出一种耐热革兰氏阴性和好氧细菌，命名为YIM 77520T。发现该菌株的细胞为棒状，菌落为浅米色和圆形。发现该菌株在pH 6.0-8.0（最佳，pH 7.0）和25-55 C（最佳，45 C）的0-2％（w / v）总盐（最佳，0％）存在下生长。 。唯一检测到的醌是Q-8，测定的基因组DNA G + C含量为66.9 mol％。主要脂肪酸（> 10％）被鉴定为iso-C16：0和iso-C15：0。发现磷脂由磷脂酰乙醇胺，磷脂酰甘油，二磷脂酰甘油，五个未知磷脂和三个氨基磷脂组成。基于16S rRNA基因序列分析，发现菌株YIM 77520T与嗜热乳杆菌YIM 77875T形成簇，并显示与嗜热乳杆菌YIM 77875T的最高16S rRNA基因序列相似性（96.0％）。这两个菌株形成了“ Xanthomonadaceae”家族的独特谱系。根据形态学和化学分类学特征，以及基因型数据，一种新的属为沃氏杆菌属。十一月被提议与腾冲旺氏细菌一起。十一月作为类型物种。腾冲弧菌的类型菌株。十一月是菌株YIM 77520 T（= DSM 25623T = CCTCC AB 2011152T）。此外，我们建议嗜热乳杆菌Wei等。 2012年被重新归类为嗜热沃氏杆菌梳。十一月（多型变数YIM 77875T = CCTCC AB 2012064T = KCTC 32020T）基于多相数据。

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