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首页> 外文期刊>Antonie van Leeuwenhoek: Journal of Microbiology and serology >Flavobacterium kyungheensis sp. nov., isolated from soil of a ginseng field
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Flavobacterium kyungheensis sp. nov., isolated from soil of a ginseng field

机译：庆州黄杆菌属十一月，与人参田土壤隔离

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摘要

A Gram-staining negative, strictly aerobic, motile by gliding, non-spore-forming, pale yellow pigmented and rod-shaped bacterium designated strain THG-107T was isolated from soil of a ginseng field on Ganghwa Island in the Republic of Korea and its taxonomic position was investigated by using a polyphasic study. Growth of strain THG-107T was found to occur at 4-37 C (optimum, 20-30 C), at pH 5.5-10 (optimum, pH 7.0) and in the presence of 0-1 % (w/v) NaCl (optimum, absence) on R2A agar. On the basis of 16S rRNA gene sequence similarity, strain THG-107T was shown to belong to the family Flavobacteriaceae and was related to Flavobacterium denitrificans ED5T (99.1 % similarity). The G+C content of the genomic DNA was determined to be 34.2 mol%. These results are consistent with characteristics of members of the genus Flavobacterium. The only isoprenoid quinone detected in strain THG-107T was menaquinone-6 (MK-6) and the major polyamine was identified as homospermidine (82.9 %). The major polar lipid detected was phosphatidylethanolamine and the major cellular fatty acids were identified as iso-C15:0 (26.3 %), iso-C17:0 3OH (12.6 %) and summed feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c; 11.6 %). Flexirubin-type pigments were found to be present. Strain THG-107T has β-glucosidase activity to convert ginsenosides Rb1 and Rd into Gyp17 and F2. DNA-DNA hybridization with F. denitrificans ED5T was 52 %. Strain THG-107T could be distinguished from F. denitrificans ED5T and the other species of the genus Flavobacterium by its phylogenetic and genetic distinctiveness and by several phenotypic properties. Therefore, strain THG-107T is considered to represent a novel species in the genus Flavobacterium, for which the name Flavobacterium kyungheensis sp. nov. is proposed (type strain THG-107T = KACC 16219T = LMG 26575T).
机译：从大韩民国江华岛的人参田土壤中分离出革兰氏染色阴性，严格有氧运动，通过滑动，无孢子形成，淡黄色色素和杆状细菌命名的菌株THG-107T。通过多相研究调查了生物分类位置。发现THG-107T菌株在4-37 C（最适温度20-30 C），pH 5.5-10（最适pH 7.0）和0-1％（w / v）NaCl存在下生长（最佳，无）在R2A琼脂上。基于16S rRNA基因序列相似性，显示菌株THG-107T属于黄杆菌科，并且与反硝化杆菌ED5T相关（99.1％相似性）。经测定，基因组DNA的G + C含量为34.2mol％。这些结果与黄杆菌属成员的特征一致。在THG-107T菌株中唯一检测到的类异戊二烯醌是甲萘醌-6（MK-6），主要多胺被鉴定为高精胺（82.9％）。检测到的主要极性脂质为磷脂酰乙醇胺，主要细胞脂肪酸被鉴定为iso-C15：0（26.3％），iso-C17：0 3OH（12.6％）并累加特征3（包含C16：1ω7c和/或C16） ：1ω6c; 11.6％）。发现存在柔红红素型颜料。 THG-107T菌株具有β-葡萄糖苷酶活性，可将人参皂苷Rb1和Rd转化为Gyp17和F2。与反硝化镰刀菌ED5T的DNA-DNA杂交率为52％。 THG-107T菌株可以通过系统发育和遗传特征以及几种表型特性与F. denitrificans ED5T和黄杆菌属的其他物种区分开。因此，菌株THG-107T被认为代表黄杆菌属中的一种新物种，其名称为黄杆菌。十一月建议（型号THG-107T = KACC 16219T = LMG 26575T）。

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