...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Animal biology >Characterization of MC1R in silky fowl,a special black-bone rooster in China
【24h】

Characterization of MC1R in silky fowl,a special black-bone rooster in China

机译：中国特有黑公鸡乌骨鸡中MC1R的特征

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The melanocortin 1 receptor (MCIR) plays an important role in determining plumage color, and the variants of MC have been found to be associated with the color of plumage and skin in both do-mestic and wild birds. However, the molecular and genetic mechanism for plumage color variation has not been reported in silky fowl, which is a unique subspecies in China with high nutritive value. We sequenced and analyzed the encoding region of MC1R from silky fowl. The predicted coding region of MC1R is 945 bp, which is the same size as the one in Gallus gallus. Six nucleotide poly-morphisms that lead to four protein mutations were detected, which were M7 I T, E92K, S 124G and H215P, respectively. Among the four mutations, the S124G mutation is found to be unique to silky fowl. A phylogenetic tree analysis of MC from silky fowl and other species of chicken shows a close relationship between silky fowl and Gallus gallus. Furthermore, the eukaryotic expression vector pEGFP-NI-MCIR was constructed, and transfected into goat fibroblasts by means of electro-poration. The success of MC1R gene expression in transfected goat fibroblasts makes it possible to develop transgenic animals for further studies.
机译：黑皮质素1受体（MCIR）在确定羽毛颜色中起重要作用，并且已经发现MC的变体与家禽和野禽中的羽毛和皮肤颜色有关。然而，尚未发现丝质家禽羽毛颜色变化的分子和遗传机制，这是中国具有很高营养价值的独特亚种。我们对丝状禽类的MC1R编码区进行了测序和分析。 MC1R的预测编码区为945 bp，与鸡鸡的大小相同。检测到导致四个蛋白质突变的六个核苷酸多态性，分别为M7 I T，E92K，S 124G和H215P。在这四个突变中，发现S124G突变是柔滑家禽特有的。用家禽和其他种鸡的MC进行系统树分析表明，家禽和鸡鸡之间存在密切的关系。此外，构建了真核表达载体pEGFP-NI-MCIR，并通过电穿孔将其转染到山羊成纤维细胞中。 MC1R基因在转染的山羊成纤维细胞中表达的成功使开发转基因动物用于进一步研究成为可能。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号