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首页> 外文期刊>Animal Biotechnology >Genetic polymorphisms in the bovine toll-like receptor 4 (TLR4) and monocyte chemo attractant protein-1(CCL2) genes: SNPs distribution analysis in Bos indicus Sahiwal cattle breed.
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Genetic polymorphisms in the bovine toll-like receptor 4 (TLR4) and monocyte chemo attractant protein-1(CCL2) genes: SNPs distribution analysis in Bos indicus Sahiwal cattle breed.

机译：牛toll样受体4（TLR4）和单核细胞化学引诱剂蛋白1（CCL2）基因的遗传多态性：Bos indicus Sahiwal牛品种中的SNP分布分析。

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摘要

Toll-like receptor 4 gene (TLR4) that recognizes the Gram negative bacterial ligand LPS was sequenced in the Bos indicus Sahiwal cattle breed. Ninety four single nucleotide polymorphisms (SNPs) were detected within 10.8 kb gene region. Seventeen of the SNPs were in the coding regions and the one at position 9589(A > G) in exon3 resulted in an amino acid change from Valine to Isoleucine. These SNPs led to generation of 27 TLR4 gene haplotypes. All the Sahiwal animals studied presently showed the occurrence of the genotype CC at gene position 9662, which codes for the amino acid threonine at position 674 of the TLR4 protein, and which had been reported to be associated with lower somatic cell score and, therefore, a lower susceptibility to mastitis, in Taurus cattle. This nucleotide configuration of the Toll-like receptor 4 gene of the Bos indicus Sahiwal cattle breed could possibly indicate toward a lower susceptibility to mastitis in the Sahiwal animals. Monocyte chemo-attractant protein-1 (CCL2) gene encoding for small inducible cytokine A2 that belongs to the CC chemokine family was also sequence characterized in these Sahiwal animals. The CCL2 gene was observed to have 12 polymorphic sites in 3.3 kb region of which one SNP at position 2500 (A > G) in exon 3 resulted in amino acid change from Valine to Isoleucine at position 46 of the mature CCL2 peptide. Seventeen haplotypes of the CCL2 gene were predicted corresponding to 12 genotypes detected.
机译：在Bos indicus Sahiwal牛品种中对识别革兰氏阴性细菌配体LPS的Toll样受体4基因（TLR4）进行了测序。在10.8 kb基因区域内检测到94个单核苷酸多态性（SNP）。 17个SNP位于编码区，外显子3中9589位（A> G）的一个导致氨基酸从缬氨酸变为异亮氨酸。这些SNP导致生成27个TLR4基因单倍型。目前研究的所有Sahiwal动物均显示在基因位置9662出现CC基因型，该基因型编码TLR4蛋白在位置674处的氨基酸苏氨酸，据报道与体细胞评分较低有关，因此，在金牛座牛中，乳腺炎的敏感性较低。 Bos indicus Sahiwal牛品种的Toll样受体4基因的这种核苷酸构型可能表明Sahiwal动物对乳腺炎的敏感性降低。在这些Sahiwal动物中，还对编码属于CC趋化因子家族的小型诱导型细胞因子A2的单核细胞趋化蛋白1（CCL2）基因进行了序列表征。观察到CCL2基因在3.3kb区域具有12个多态性位点，其中外显子3中2500位（A＞ G）的一个SNP导致成熟CCL2肽的46位氨基酸从缬氨酸变为异亮氨酸。预测对应于检测到的12个基因型的CCL2基因的十七个单倍型。

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