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首页> 外文期刊>EMBO Journal >The PITSLRE/CDK11(p58) protein kinase promotes centrosome maturation and bipolar spindle formation
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The PITSLRE/CDK11(p58) protein kinase promotes centrosome maturation and bipolar spindle formation

机译：PITSLRE / CDK11 (p58)蛋白激酶促进中心体成熟和双极纺锤体形成

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摘要

The CDK11 (cyclin-dependent kinase 11) gene has an internal ribosome entry site ( IRES), allowing the expression of two protein kinases. The longer 110-kDa isoform is expressed at constant levels during the cell cycle and the shorter 58-kDa isoform is expressed only during G2 and M phases. By means of RNA interference (RNAi), we show that the CDK11 gene is required for mitotic spindle formation. CDK11 RNAi leads to mitotic checkpoint activation. Mitotic cells are arrested with short or monopolar spindles. gamma- Tubulin as well as Plk1 and Aurora A protein kinase levels are greatly reduced at centrosomes, resulting in microtubule nucleation defects. We show that the mitotic CDK11p58 isoform, but not the CDK11p110 isoform, associates with mitotic centrosomes and rescues the phenotypes resulting from CDK11 RNAi. This work demonstrates for the first time the role of CDK11p58 in centrosome maturation and bipolar spindle morphogenesis.
机译：CDK11(细胞周期蛋白依赖性激酶11)基因有一个内部核糖体进入站点(IRES),允许两个蛋白激酶的表达。110 kda同种型表达在恒定水平在细胞周期和较短的58-kDa同种型表达只在G2和M阶段。通过RNA干扰(RNAi),我们证明有丝分裂纺锤体所需CDK11基因形成。激活。或单极纺锤波。Plk1和极光蛋白激酶水平在中心体大大减少,导致微管成核的缺陷。有丝分裂CDK11p58同种型,但不是CDK11p110同种型,同事和有丝分裂中心体救助CDK11 RNAi的表型结果。这演示了第一次工作CDK11p58中心体成熟和的作用双极纺锤体形态发生。

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