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首页> 外文期刊>Applied Microbiology and Biotechnology >Glycerol-based sterilization bioindicator system from Bacillus atrophaeus: Development, performance evaluation, and cost analysis
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Glycerol-based sterilization bioindicator system from Bacillus atrophaeus: Development, performance evaluation, and cost analysis

机译：萎缩芽孢杆菌甘油为基础的杀菌生物指示剂系统：开发，性能评估和成本分析

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摘要

The development of new value-added applications for glycerol is of worldwide interest because of the environmental and economic problems that may be caused by an excess of glycerol generated from biodiesel production. A novel use of glycerol as a major substrate for production of a low-cost sterilization biological indicator system (BIS; spores on a carrier plus a recovery medium) was investigated. A sequential experimental design strategy was applied for product development and optimization. The proposed recovery medium enables germination and outgrowth of heat-damaged spores, promoting a D 160 C value of 6.6 ± 0.1 min. Bacillus atrophaeus spores production by solid-state fermentation reached a 2.3 ± 1.2 × 10~8 CFU/g dry matter. Sporulation kinetics results allowed this process to be restricted in 48 h. Germination kinetics demonstrated the visual identification of nonsterile BIS within 24 h. Performance evaluation of the proposed BIS against dry-heat and ethylene oxide sterilization showed compliance with the regulatory requirements. Cost breakdowns were from 41.8 (quality control) up to 72.8 % (feedstock). This is the first report on sterilization BIS production that uses glycerol as a sole carbon source, with significant cost reduction and the profitable use of a biodiesel byproduct.
机译：由于生物柴油生产中产生的过量甘油可能引起环境和经济问题，因此甘油的新的增值应用的开发引起了全世界的关注。研究了将甘油用作生产低成本灭菌生物指示剂系统（BIS;载体上的孢子加上回收介质）的主要底物的新颖用途。顺序实验设计策略用于产品开发和优化。拟议的回收介质能够使受热损伤的孢子萌发和生长，从而使D 160 C值为6.6±0.1分钟。固态发酵生产的萎缩芽孢杆菌孢子达到2.3±1.2×10〜8 CFU / g干物质。孢子形成动力学结果使该过程限制在48小时内。发芽动力学证明了24小时内非无菌BIS的视觉识别。拟议的BIS对干热和环氧乙烷灭菌的性能评估表明符合法规要求。成本细分从41.8（质量控制）到72.8％（原料）。这是有关使用甘油作为唯一碳源的灭菌BIS生产的第一份报告，该报告显着降低了成本，并有利地利用了生物柴油副产品。

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