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首页> 外文期刊>Applied Microbiology and Biotechnology >Symbiotic efficiency and phylogeny of the rhizobia isolated from Leucaena leucocephala in arid-hot river valley area in Panxi, Sichuan, China
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Symbiotic efficiency and phylogeny of the rhizobia isolated from Leucaena leucocephala in arid-hot river valley area in Panxi, Sichuan, China

机译：四川攀西干旱热河谷地区银合欢根瘤菌的共生效率和系统发育

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摘要

In search of effective nitrogen-fixing strains for inoculating Leucaena leucocephala, we assessed the symbiotic efficiency of 41 rhizobial isolates from root nodules of L. leucocephala growing in the arid-hot river valley area in Panxi, China. The genetic diversity of the isolates was studied by analyzing the housekeeping genes 16S rRNA and recA, and the symbiotic genes nifH and nodC. In the nodulation and symbiotic efficiency assay, only 11 of the 41 isolates promoted the growth of L. leucocephala while the majority of the isolates were ineffective in symbiotic nitrogen fixation. Furthermore, one fourth of the isolates had a growth slowing effect on the host. According to the 16S rRNA and recA gene analyses, most of the isolates were Ensifer spp. The remaining isolates were assigned to Rhizobium, Mesorhizobium and Bradyrhizobium. The sequence analyses indicated that the L. leucocephala rhizobia had undergone gene recombination. In contrast to the promiscuity observed as a wide species distribution of the isolates, the results implied that L. leucocephala is preferentially nodulated by strains that share common symbiosis genes. The symbiotic efficiency was not connected to chromosomal background of the symbionts and isolates carrying a similar nifH or nodC showed totally different nitrogen fixation efficiency.
机译：为了寻找有效的固氮菌接种白斑白术，我们评估了攀西干旱热河谷地区生长的白斑白头翁根瘤中41种根瘤菌的共生效率。通过分析管家基因16S rRNA和recA以及共生基因nifH和nodC来研究分离株的遗传多样性。在结瘤和共生效率测定中，41个分离株中只有11个促进白头球菌的生长，而大多数分离株对共生固氮无效。此外，四分之一的分离株对宿主具有生长减慢作用。根据16S rRNA和recA基因分析，大多数分离株是Ensifer spp。其余的分离物被分为根瘤菌，中生根瘤菌和缓生根瘤菌。序列分析表明，白带状根瘤菌已经进行了基因重组。与作为分离物的广泛物种分布所观察到的滥交相反，该结果暗示白带乳头状菌优先被共享共有共生基因的菌株所致。共生效率与共生体的染色体背景无关，携带相似nifH或nodC的分离株显示出完全不同的固氮效率。

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