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首页> 外文期刊>Applied Microbiology and Biotechnology >Improvement of transglutaminase production by extending differentiation phase of Streptomyces hygroscopicus: mechanism and application
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Improvement of transglutaminase production by extending differentiation phase of Streptomyces hygroscopicus: mechanism and application

机译：通过延长吸水链霉菌的分化期来提高转谷氨酰胺酶的产生：机理和应用

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摘要

Streptomyces transglutaminase (TGase) is an important industrial enzyme that catalyzes cross-linking of proteins. It is secreted as a zymogene and then is activated by proteases under physiological conditions. Although the activation process of TGase has been well investigated, the physiological function of TGase in Streptomyces has not been revealed. In this study, physiological function of TGase from Streptomyces hygroscopicus was found to be involved in differentiation by construction of a TGase gene interruption mutation strain (Δtg). The mutant Δtg showed an absence of differentiation compared with the parent strain. Furthermore, the production of TGase was found to be increased with the extending growth arrest phase of mycelium in submerged cultures. Thus, to enhance yield of TGase, the mycelium differentiation of Streptomyces was regulated via low temperature stress in a 3-L stirredtank fermenter. The production of TGase increased by 39 % through extending the growth arrest phase for 4 h. This study found that TGase is involved in Streptomyces differentiation and proposed an approach to improve TGase production by regulation of mycelium differentiation in submerged cultures.
机译：链霉菌转谷氨酰胺酶（TGase）是一种重要的工业酶，可催化蛋白质的交联。它被分泌为酶基因，然后在生理条件下被蛋白酶激活。尽管已经很好地研究了TGase的激活过程，但是尚未揭示TGase在链霉菌中的生理功能。在这项研究中，发现吸水链霉菌的TGase的生理功能与TGase基因中断突变菌株（Δtg）的构建有关。与亲本菌株相比，突变体Δtg显示出没有分化。此外，发现淹没培养物中菌丝体的生长停滞期延长，TGase的产量增加。因此，为了提高TGase的产量，链霉菌的菌丝体分化是通过在3-L搅拌罐发酵罐中通过低温胁迫来调节的。通过延长生长停滞期4小时，TGase的产量增加了39％。这项研究发现TGase参与链霉菌的分化，并提出了一种通过调节淹没培养物中菌丝体分化来提高TGase产量的方法。

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