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首页> 外文期刊>Applied Microbiology and Biotechnology >Glucoamylase production in batch, chemostat and fed-batch cultivations by an industrial strain of Aspergillus niger
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Glucoamylase production in batch, chemostat and fed-batch cultivations by an industrial strain of Aspergillus niger

机译：黑曲霉工业菌株分批，恒化和分批补料生产的葡糖淀粉酶

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The Aspergillus niger strain BO-1 was grown in batch, continuous (chemostat) and fed-batch cultivations in order to study the production of the extracellular enzyme glucoamylase under different growth conditions. In the pH range 2.5-6.0, the specific glucoamylase productivity and the specific growth rate of the fungus were independent of pH when grown in batch cultivations. The specific glucoamylase productivity increased linearly with the specific growth rate in the range 0-0.1 h(-1) and was constant in the range 0.1-0.2 h(-1) Maltose and maltodextrin were non-inducing carbon sources compared to glucose, and the maximum specific growth rate was 0.19 +/- 0.02 h(-1) irrespective of whether glucose or maltose was the carbon source. In fed-batch cultivations, glucoamylase titres of up to 6.5 g 1(-1) were obtained even though the strain contained only one copy of the glaA gene. [References: 23]
机译：为了研究在不同生长条件下细胞外酶葡糖淀粉酶的产生，黑曲霉菌株BO-1分批，连续（化学稳定剂）和分批补料培养。在分批培养中生长时，在2.5-6.0的pH范围内，真菌的比葡糖淀粉酶生产率和比生长速率与pH无关。比葡糖淀粉酶的生产率随比增长率在0-0.1 h（-1）范围内线性增加，并在0.1-0.2 h（-1）范围内恒定。与葡萄糖相比，麦芽糖和麦芽糊精是非诱导性碳源，并且无论葡萄糖或麦芽糖是碳源，最大比生长速率均为0.19 +/- 0.02 h（-1）。在分批补料培养中，即使该菌株仅包含一个拷贝的glaA基因，其葡糖淀粉酶滴度仍可高达6.5 g 1（-1）。 [参考：23]

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