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首页> 外文期刊>Applied Microbiology and Biotechnology >Production of sophorolipids with enhanced volumetric productivity by means of high cell density fermentation
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Production of sophorolipids with enhanced volumetric productivity by means of high cell density fermentation

机译：通过高细胞密度发酵生产槐糖脂，提高了产量

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摘要

To achieve high time-space efficiency for sophorolipid production with yeast Candida bombicola, a strategy of high cell density fermentation was employed. The approach consisted of two sequential stages: (1) the optimization of the carbon source and the nutrient concentration to achieve the maximal cell density and (2) the computer-aided adjustment of physical parameters and the controlled feeding of substrates for enhanced volumetric productivity. Both stages have been successfully implemented in a 10-L fermenter, where up to 80 g dry cell weight/L was obtained and a remarkably high volumetric productivity (> 200 g isolated sophorolipids/L/day) was achieved. Both the biomass and volumetric productivity were markedly higher than previously reported. Specifically, the high productivity of sophorolipids could be attained on a very short time scale (24 h), highlighting the industrial potential of the platform developed in this work.
机译：为了获得高效率的时空效率，利用酵母念珠菌生产槐糖脂，采用了高细胞密度发酵的策略。该方法包括两个连续的阶段：（1）优化碳源和营养物浓度以实现最大的细胞密度;（2）计算机辅助调整的物理参数和受控的底物进料，以提高容积生产率。这两个阶段均已在10 L发酵罐中成功实施，该发酵罐中的干细胞重高达80 g / L，并且具有显着高的容积生产率（> 200 g分离的槐糖脂/ L /天）。生物量和体积生产率均显着高于以前报道的水平。具体而言，槐糖脂的高生产率可以在非常短的时间内（24小时）获得，这突出表明了这项工作开发的平台的工业潜力。

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