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首页> 外文期刊>Applied Microbiology and Biotechnology >Effects of sugar and amino acid supplementation on Aureobasidium pullulans NRRL 58536 antifungal activity against four Aspergillus species
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Effects of sugar and amino acid supplementation on Aureobasidium pullulans NRRL 58536 antifungal activity against four Aspergillus species

机译：糖和氨基酸的添加对支链霉菌NRRL 58536的抗真菌活性对四种曲霉的影响

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摘要

Cultured cell extracts from ten tropical strains of Aureobasidium pullulans were screened for antifungal activity against four pathogenic Aspergillus species (Aspergillus flavus, Aspergillus niger, Aspergillus fumigatus, and Aspergillus terreus) using the well diffusion and conidial germination inhibition assays. The crude cell extract from A. pullulans NRRL 58536 resulted in the greatest fungicidal activity against all four Aspergillus species and so was selected for further investigation into enhancing the production of antifungal activity through optimization of the culture medium, carbon source (sucrose and glucose) and amino acid (phenylalanine, proline, and leucine) supplementation. Sucrose did not support the production of any detectable antifungal activity, while glucose did with the greatest antifungal activity against all four Aspergillus species being produced in cells grown in medium containing 2.5% (w/v) glucose.With respect to the amino acid supplements, variable trends between the different Aspergillus species and amino acid combinations were observed, with the greatest antifungal activities being obtained when grown with phenylalanine plus leucine supplementation for activity against A. flavus, proline plus leucine for A. terreus, and phenylalanine plus proline and leucine for A. niger and A. fumigatus. Thin layer chromatography, spectrophotometry, high-performance liquid chromatography, ~1H-nuclear magnetic resonance, and MALDI-TOF mass spectrometry analyses were all consistent with the main component of the A. pullulans NRRL 58536 extracts being aureobasidins.
机译：使用孔扩散和分生孢子萌发抑制试验，从十种热带金黄色葡萄球菌菌株的培养细胞提取物中筛选出针对四种病原性曲霉菌（黄曲霉，黑曲霉，烟曲霉和土曲霉）的抗真菌活性。 A. Pullulans NRRL 58536的粗细胞提取物对所有四种曲霉菌均具有最大的杀真菌活性，因此被选作进一步研究，以通过优化培养基，碳源（蔗糖和葡萄糖）和抗微生物剂来增强抗真菌活性。氨基酸（苯丙氨酸，脯氨酸和亮氨酸）的补充。蔗糖不支持产生任何可检测到的抗真菌活性，而葡萄糖对在含2.5％（w / v）葡萄糖的培养基中生长的细胞中产生的所有四种曲霉菌具有最大的抗真菌活性。观察到不同曲霉菌种和氨基酸组合之间的变化趋势，当与苯丙氨酸加亮氨酸一起补充对黄曲霉的活性，脯氨酸加亮氨酸对土壤曲霉有活性，苯丙氨酸加脯氨酸和亮氨酸对苯丙氨酸有活性时，获得最大的抗真菌活性。黑曲霉和烟曲霉。薄层色谱法，分光光度法，高效液相色谱法，〜1H核磁共振和MALDI-TOF质谱分析均与金黄色葡萄球菌NRRL 58536提取物的主要成分一致。

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