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首页> 外文期刊>Applied Microbiology and Biotechnology >Identification of Rhodococccus erythropolis isolates capable of degrading the fungicide carbendazim
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Identification of Rhodococccus erythropolis isolates capable of degrading the fungicide carbendazim

机译：能够降解杀菌剂多菌灵的红球红球菌分离株的鉴定

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Continuous enrichment cultures were used to identify bacterial isolates capable of degrading the fungicide carbendazim. The bacteria originated from sites that had been repeatedly treated with the structurally related parent fungicide, benomyl, over a period of several years. Six isolates were identified as carbendazim degraders on the basis of their ability to produce diffusion-clearing zones on a minimal sails solid medium spray-coated with a 0.1% solution of carbendazim, their ability to grow in a minimal salts broth supplemented with carbendazim as the sole carbon source, and their ability to reduce carbendazim levels in liquid cultures. All six isolates were identified as Rhodococcus erythropolis or a closely related species by analyses of nutritional utilization and whole-cell fatty acid methyl ester profiles. A chemically induced mutant of R. erythropolis isolate B2E was identified that was no longer capable of degrading carbendazim, as determined by negative results in all three assays. Further characterization of these strains provides an opportunity for their development in bioremediation of the compound.
机译：连续富集培养用于鉴定能够降解杀菌剂多菌灵的细菌分离株。细菌起源于经过数年的反复使用与结构相关的母体杀真菌剂苯菌灵处理过的位点。根据六个分离物的识别能力，根据其在喷有0.1％多菌灵溶液的最小风帆固体培养基上产生扩散清除区的能力，以及它们在补充了多菌灵的最小盐汤中生长的能力。唯一的碳源，以及它们降低液体培养物中多菌灵水平的能力。通过营养利用和全细胞脂肪酸甲酯分析，将所有六个分离株鉴定为红球菌或密切相关的物种。如在所有三个测定中的阴性结果所确定的，鉴定出化学诱导的红细菌分离株B2E的突变体不再能够降解多菌灵。这些菌株的进一步表征为其在化合物的生物修复中的发展提供了机会。

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