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The diverse and expanding role of mass spectrometry in structural and molecular biology

机译:质量的多样化和扩大的作用光谱法在结构和分子生物学

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摘要

The emergence of proteomics has led to major technological advances in mass spectrometry (MS). These advancements not only benefitted MS-based high-throughput proteomics but also increased the impact of mass spectrometry on the field of structural and molecular biology. Here, we review how state-of-the-art MS methods, including native MS, top-down protein sequencing, cross-linking-MS, and hydrogen-deuterium exchange-MS, nowadays enable the characterization of biomolecular structures, functions, and interactions. In particular, we focus on the role of mass spectrometry in integrated structural and molecular biology investigations of biological macromolecular complexes and cellular machineries, highlighting work on CRISPR-Cas systems and eukaryotic transcription complexes.
机译:蛋白质组学的出现导致了专业技术进步在质谱(MS)。这些进步不仅受益MS-based高通量蛋白质组学也增加了质谱分析的领域的影响结构和分子生物学。女士如何先进的方法,包括本地女士,自上而下的蛋白质测序,cross-linking-MS, hydrogen-deuteriumexchange-MS,如今使表征生物分子的结构、功能和交互。质谱的结构和集成分子生物学调查的生物大分子复合物和细胞机械,凸显CRISPR-Cas工作系统和真核转录复合体。

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