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首页> 外文期刊>Applied Microbiology and Biotechnology >Characterization of three novel desaturases involved in the delta-6 desaturation pathways for polyunsaturated fatty acid biosynthesis from Phytophthora infestans
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Characterization of three novel desaturases involved in the delta-6 desaturation pathways for polyunsaturated fatty acid biosynthesis from Phytophthora infestans

机译：疫霉疫霉多不饱和脂肪酸生物合成的delta-6去饱和途径中涉及的三种新型去饱和酶的表征

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摘要

Phytophthora infestans is the causative agent of potato blight that resulted in the great famine in Ireland in the nineteenth century. This microbe can release large amounts of the C20 very long-chain polyunsaturated fatty acids arachidonic acid (ARA; 20:4Δ~(5, 8, 11, 14)) and eicosapentaenoic acid (EPA; 20:5Δ~(5, 8, 11, 14, 17)) upon invasion that is known to elicit a hypersensitive response to their host plant. In order to identify enzymes responsible for the biosynthesis of these fatty acids, we blasted the recently fully sequenced P. infestans genome and identified three novel putatively encoding desaturase sequences. These were subsequently functionally characterized by expression in Saccharomyces cerevisiae and confirmed that they encode desaturases with Δ12, Δ6 and Δ5 activity, designated here as PinDes12, PinDes6 and PinDes5, respectively. This, together with the combined fatty acid profiles and a previously identified Δ6 elongase activity, implies that the ARA and EPA are biosynthesized predominantly via the Δ6 desaturation pathways in P. infestans. Elucidation of ARA and EPA biosynthetic mechanism may provide new routes to combating this potato blight microbe directly or by means of conferring resistance to important crops.
机译：疫病疫霉是造成马铃薯疫病的病原体，在19世纪引起了爱尔兰的饥荒。该微生物可以释放大量C20非常长链的多不饱和脂肪酸花生四烯酸（ARA; 20：4Δ〜（5，8，11，14））和二十碳五烯酸（EPA; 20：5Δ〜（5，8， 11、14、17））时，已知会引起对其寄主植物的过敏反应。为了鉴定负责这些脂肪酸生物合成的酶，我们筛选了最近完全测序的疫霉菌基因组，并鉴定了三个新颖的假定编码去饱和酶序列。随后通过在酿酒酵母中的表达对它们进行功能表征，并证实它们编码具有Δ12，Δ6和Δ5活性的去饱和酶，在此分别命名为PinDes12，PinDes6和PinDes5。这与组合的脂肪酸谱和先前确定的Δ6延长酶活性一起，意味着ARA和EPA主要通过感染晚疫病菌中的Δ6去饱和途径进行生物合成。阐明ARA和EPA的生物合成机制可能会提供直接或通过赋予重要农作物抗性的方式来对抗马铃薯白叶枯病微生物的新途径。

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